IFN gamma Mouse ProQuantum Immunoassay Kit,IFN gamma Mouse ProQuantum Immunoassay Kit/小鼠IFNγ ProQuantum免疫分析试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

IFN gamma Mouse ProQuantum Immunoassay Kit,IFN gamma Mouse ProQuantum Immunoassay Kit/小鼠IFNγ ProQuantum免疫分析试剂盒

2024-10-23

IFN gamma Mouse ProQuantum Immunoassay Kit/小鼠IFNγ ProQuantum免疫分析试剂盒

货号：A41150,A411505

规格：96 Tests,5X96 Tests

价格：6561,29500

产品类型：Proquantum ELISA

品牌：Thermo Fisher

物种：小鼠

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	<0.05 pg/mL
检测范围	0.064 – 5,000 pg/mL
样本类型/体积	血浆 2-5 μL 血清 2-5 μL 上清液 2-5 μL
操作时间	30 min
测试时间	2 hr
Homogenous (no wash)	Yes
仪器设备	qPCR Instruments
规格	5 X 96 Tests
组份	- 分析稀释缓冲液 - 抗体结合物 A - 抗体结合物 B - 抗体结合稀释缓冲液 - 标准品 - 预混料 - 连接酶
储存温度	-20℃
运输条件	干冰
Protein name	IFN gamma
Protein aliases	gamma interferon, IFN-gamma, Immune interferon, Interferon gamma
物种（Tested）	小鼠
试剂盒类型	Proximity-based Immunoassay
标记（染料）	FAM
Gene ID	(Mouse) 15978
Gene aliases	Ifg, IFN-g, Ifng
Gene symbol	Ifng
UniProt ID	(Mouse) P01580

The ProQuantum Mouse IFN gamma Immunoassay Kit is designed to provide highly sensitive quantitative measurements of mouse IFN gamma protein in small sample volumes. Utilizing proximity ligation assay (PLA) technology, the assay combines the analyte specificity of high-affinity antibody-antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a simple yet powerful next-generation protein quantitation platform. A user-friendly workflow combined with intuitive Cloud-based software for analytics enables sample-to-answer in just 2 hours.ProQuantum小鼠IFNγ免疫分析试剂盒用于提供小样品量的小鼠IFNγ蛋白的高灵敏度定量测量。利用邻近连接测定（PLA）技术，该测定结合了高亲和力抗体-抗原结合的分析物特异性与实时PCR的信号检测和扩增功能，从而实现了一个简单而强大的下一代蛋白质定量平台。用户友好的工作流程与直观的基于云的软件相结合进行分析，可在2小时内完成从样品到答案的分析。ProQuantum immunoassays utilize a matched pair of target-specific antibodies, each conjugated to a DNA oligonucleotide. During antibody-analyte binding, the two DNA oligos are brought into close proximity, which allows for ligation of the two strands and subsequent creation of a template strand for amplification. This platform leverages the sensitivity and large dynamic range of Applied Biosystems TaqMan real-time PCR technology.ProQuantum免疫测定法使用一对配对的靶标特异性抗体，每种抗体均与DNA寡核苷酸偶联。在抗体-分析物结合过程中，两个DNA寡核苷酸紧密靠近，这允许两条链连接并随后产生用于扩增的模板链。该平台利用了Applied Biosystems TaqMan实时PCR技术的灵敏度和大动态范围。The assay workflow is fast and easy—2 steps in 2 hours. There are a total of 7 components in each kit (figure below). First, mix the antibody-conjugates, dilute the sample, and create the standard curve in a working plate. Then, using a multi-channel pipette, add the antibody-conjugates and sample (or standard) into the wells of a PCR plate and incubate for 1 hour. Combine the master mix and ligase and add to the wells of the PCR plate, then run the plate on any qPCR instrument. After the run is complete, import the results file into the ProQuantum cloud-based software at https://apps.thermofisher.com/apps/proquantum. Using this software, the data can be analyzed easily to obtain protein concentration values. The software allows you to set up standard curves, design plate layouts, set up customized assay instructions, and obtain robust statistical group-wise comparisons.测定工作流程快速简便— 2小时内完成2个步骤。每个试剂盒中总共有7个组份。首先，混合抗体-缀合物，稀释样品，并在工作板上创建标准曲线。然后，使用多通道移液器，将抗体结合物和样品（或标准品）添加到PCR板的孔中，并孵育1小时。合并预混液和连接酶，并添加到PCR板的孔中，然后在任何qPCR仪器上运行该板。运行完成后，将结果文件导入https://apps.thermofisher.com/apps/proquantum的基于ProQuantum云的软件中。使用此软件，可以轻松分析数据以获得蛋白质浓度值。该软件允许您设置标准曲线，设计板布局，设置定制化验说明并获得可靠的逐组统计比较。Target information 靶标信息IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.IFNγ（干扰素γ，II型干扰素）是一种巨噬细胞活化因子，是一种免疫干扰素，主要由T淋巴细胞和自然杀伤细胞响应抗原，促分裂原，葡萄球菌肠毒素B，植物血凝素和其他细胞因子而产生。 IFNγ是由两个146个氨基酸亚基组成的二聚体蛋白。 IFNγ是一种糖蛋白，功能上以约45 kDa的同型二聚体形式存在。在SDS-PAGE上，由于差异糖基化，IFNγ出现了25、20和15.5 kDa较小的条带。 IFNγ同二聚体的生物活性是高度物种特异性的。人IFNγ与小鼠无交叉反应。 IFNγ功能包括以下各项：抗病毒活性，肿瘤抗增殖活性，I类和II类MHC的诱导，巨噬细胞活化以及B淋巴细胞增强的免疫球蛋白分泌。 IFNγ参与细胞因子的调节，并与其他细胞因子协同作用。 IFNγ的激活是通过IFNγ受体I和II的结合并激活JAK-STAT途径进行的。 IFNγ与IFNα或IFNβ没有任何同源性，但是人IFNγ与小鼠IFNγ具有约40％的序列同源性。 IFNγ被IL2，FGF碱性蛋白，EGF上调，而维生素D3或DMN被下调。 IFNγ基因突变与再生障碍性贫血有关。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

特点：

- 高灵敏度 - 更高的灵敏度检测低水平的蛋白质- 宽动态范围- 样品消耗少 - 使用2–5 µL样品- 快速，轻松的工作流程 - 从样品到取样仅需2个小时，无需清洗- 无需购买专有仪器 - 可在任何实时PCR仪器上运行

数据：

IFN gamma Mouse ProQuantum Immunoassay KitHow ProQuantum immunoassays work. (A) Antibody-antigen binding: Antibodies bind to two separate epitopes on the antigen (during a 1-hr incubation), which brings the two conjugated oligonucleotides into close proximity. (B) Ligation and amplification of signal (in a qPCR instrument): DNA ligase and a third splint oligonucleotide connector are added to ligate the two ends of the conjugated oligonucleotides, creating a 100-base DNA template. Once the ligase is inactivated at 95°C, the sample is amplified through 40 cycles of annealing and extension. The amount of DNA produced is measured after each amplification cycle via fluorescent dyes, which exhibit fluorescence that is directly proportional to the number of PCR product molecules (or amplicons) generated. This graph shows the fluorescence vs. cycle number curves for four different starting protein concentrations. The dashed line represents the fluorescence threshold. The cycle number required to reach the fluorescence threshold (threshold cycle or Ct) is plotted vs. protein concentration to create a standard curve.ProQuantum免疫测定如何工作。（A）抗体-抗原结合：抗体与抗原上的两个独立表位结合（在1小时的孵育过程中），这使两个结合的寡核苷酸紧密相邻。（B）信号的连接和扩增（在qPCR仪器中）：添加DNA连接酶和第三个夹板寡核苷酸连接物以连接缀合的寡核苷酸的两端，从而产生100个碱基的DNA模板。连接酶在95°C失活后，样品会通过40个退火和延伸循环进行扩增。在每个扩增循环后，通过荧光染料测量产生的DNA量，该荧光染料显示的荧光与产生的PCR产物分子（或扩增子）的数量成正比。该图显示了四种不同起始蛋白质浓度的荧光与循环数曲线。虚线表示荧光阈值。将达到荧光阈值所需的循环数（阈值循环或Ct）对蛋白质浓度作图，以创建标准曲线。

IFN gamma Mouse ProQuantum Immunoassay Kit<b>ProQuantum kit contents</b><br />Components are color coded for ease of use.

技术参数

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IFN gamma Mouse ProQuantum Immunoassay Kit,IFN gamma Mouse ProQuantum Immunoassay Kit/小鼠IFNγ ProQuantum免疫分析试剂盒

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