MCP-1 Human ProQuantum Immunoassay Kit,MCP-1 Human ProQuantum Immunoassay Kit/人MCP-1 ProQuantum免疫测定试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

MCP-1 Human ProQuantum Immunoassay Kit,MCP-1 Human ProQuantum Immunoassay Kit/人MCP-1 ProQuantum免疫测定试剂盒

2024-10-23

MCP-1 Human ProQuantum Immunoassay Kit/人MCP-1 ProQuantum免疫测定试剂盒

货号：A35598,A355985

规格：96 Tests,5X96 Tests

价格：6561,29500

产品类型：Proquantum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	<0.05 pg/mL
检测范围	0.064-5000
样本类型/体积	血浆 2-5 μL 血清 2-5 μL 上清液 2-5 μL
操作时间	30 min
测试时间	2 hr
Homogenous (no wash)	Yes
仪器设备	qPCR Instruments
规格	5 X 96 Tests
组份	- 分析稀释缓冲液 - 抗体结合物 A - 抗体结合物 B - 抗体结合稀释缓冲液 - 标准品 - 预混料 - 连接酶
储存温度	-20℃
运输条件	干冰
Protein name	CCL2 (MCP-1)
Protein aliases	C-C motif chemokine 2, chemokine (C-C motif) ligand 2, HC11, MCAF, MCP-1, Monocyte chemoattractant protein 1, monocyte chemoattractant protein-1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je), small inducible cytokine subfamily A (Cys-Cys), member 2, Small-inducible cytokine A2
物种（Tested）	人
试剂盒类型	Proximity-based Immunoassay
标记（染料）	FAM
Gene ID	(Human) 6347
Gene aliases	CCL2, GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, SCYA2, SMC-CF
Gene symbol	CCL2
UniProt ID	(Human) P13500

The ProQuantum Human MCP-1 Immunoassay Kit is designed to provide highly sensitive quantitative measurements of human MCP-1 in small sample volumes. Utilizing proximity ligation assay (PLA) technology, the assay combines the analyte specificity of high-affinity antibody-antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a simple yet powerful next-generation protein quantitation platform. A user-friendly workflow combined with intuitive Cloud-based software for analytics enables sample-to-answer in just 2 hours.ProQuantum人MCP-1免疫分析试剂盒用于提供小样品量中人MCP-1的高灵敏度定量测量。利用邻近连接测定（PLA）技术，该测定结合了高亲和力抗体-抗原结合的分析物特异性与实时PCR的信号检测和扩增功能，从而实现了一个简单而强大的下一代蛋白质定量平台。用户友好的工作流程与直观的基于云的软件相结合进行分析，可在2小时内完成从样品到答案的分析。ProQuantum immunoassays utilize a matched pair of target-specific antibodies, each conjugated to a DNA oligonucleotide. During antibody-analyte binding, the two DNA oligos are brought into close proximity, which allows for ligation of the two strands and subsequent creation of a template strand for amplification. This platform leverages the sensitivity and large dynamic range of Applied Biosystems TaqMan real-time PCR technology.ProQuantum免疫测定法使用一对配对的靶标特异性抗体，每种抗体均与DNA寡核苷酸偶联。在抗体-分析物结合过程中，两个DNA寡核苷酸紧密靠近，这允许两条链连接并随后产生用于扩增的模板链。该平台利用了Applied Biosystems TaqMan实时PCR技术的灵敏度和较大的动态范围。The assay workflow is fast and easy—2 steps in 2 hours. There are a total of 7 components in each kit (figure below). First, mix the antibody-conjugates, dilute the sample, and create the standard curve in a working plate. Then, using a multi-channel pipette, add the antibody-conjugates and sample (or standard) into the wells of a PCR plate and incubate for 1 hour. Combine the master mix and ligase and add to the wells of the PCR plate, then run the plate on any qPCR instrument. After the run is complete, import the results file into the ProQuantum cloud-based software at https://apps.thermofisher.com/apps/proquantum. Using this software, the data can be analyzed easily to obtain protein concentration values. The software allows you to set up standard curves, design plate layouts, set up customized assay instructions, and obtain robust statistical group-wise comparisons.测定工作流程快速简便— 2小时内完成2个步骤。每个试剂盒中总共有7个组份。首先，混合抗体-结合物，稀释样品，并在工作板上创建标准曲线。然后，使用多通道移液器，将抗体结合物和样品（或标准品）添加到PCR板的孔中，并孵育1小时。合并预混液和连接酶，并添加到PCR板的孔中，然后在任何qPCR仪器上运行该板。运行完成后，将结果文件导入https://apps.thermofisher.com/apps/proquantum的基于ProQuantum云的软件中。使用此软件，可以轻松分析数据以获得蛋白质浓度值。该软件允许您设置标准曲线，设计板布局，设置定制化验说明并获得可靠的逐组统计比较。Target information 靶标信息This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.该基因是聚集在17号染色体q臂上的几种细胞因子基因之一。细胞因子是涉及免疫调节和炎症过程的一系列分泌蛋白。该基因编码的蛋白质在结构上与细胞因子的CXC亚家族有关。该亚家族的成员的特征是两个半胱氨酸被一个氨基酸隔开。该细胞因子显示出对单核细胞和嗜碱性粒细胞的趋化活性，但对嗜中性粒细胞或嗜酸性粒细胞没有趋化活性。它与牛皮癣，类风湿性关节炎和动脉粥样硬化等以单核细胞浸润为特征的疾病的发病机理有关。它与趋化因子受体CCR2和CCR4结合。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

特点：

- 高灵敏度 - 更高的灵敏度检测低水平的蛋白质- 宽动态范围- 样品消耗少 - 使用2–5 µL样品- 快速，轻松的工作流程 - 从样品到取样仅需2个小时，无需清洗- 无需购买专有仪器 - 可在任何实时PCR仪器上运行

数据：

MCP-1 Human ProQuantum Immunoassay KitHow ProQuantum immunoassays work. (A) Antibody-antigen binding: Antibodies bind to two separate epitopes on the antigen (during a 1-hr incubation), which brings the two conjugated oligonucleotides into close proximity. (B) Ligation and amplification of signal (in a qPCR instrument): DNA ligase and a third splint oligonucleotide connector are added to ligate the two ends of the conjugated oligonucleotides, creating a 100-base DNA template. Once the ligase is inactivated at 95°C, the sample is amplified through 40 cycles of annealing and extension. The amount of DNA produced is measured after each amplification cycle via fluorescent dyes, which exhibit fluorescence that is directly proportional to the number of PCR product molecules (or amplicons) generated. This graph shows the fluorescence vs. cycle number curves for four different starting protein concentrations. The dashed line represents the fluorescence threshold. The cycle number required to reach the fluorescence threshold (threshold cycle or Ct) is plotted vs. protein concentration to create a standard curve.ProQuantum免疫测定如何工作。（A）抗体-抗原结合：抗体与抗原上的两个独立表位结合（在1小时的孵育过程中），这使两个结合的寡核苷酸紧密相邻。（B）信号的连接和扩增（在qPCR仪器中）：添加DNA连接酶和第三个夹板寡核苷酸连接物以连接缀合的寡核苷酸的两端，从而产生100个碱基的DNA模板。连接酶在95°C失活后，样品会通过40个退火和延伸循环进行扩增。在每个扩增循环后，通过荧光染料测量产生的DNA量，该荧光染料显示的荧光与产生的PCR产物分子（或扩增子）的数量成正比。该图显示了四种不同起始蛋白质浓度的荧光与循环数曲线。虚线表示荧光阈值。将达到荧光阈值所需的循环数（阈值循环或Ct）对蛋白质浓度作图，以创建标准曲线。

MCP-1 Human ProQuantum Immunoassay Kit<b>ProQuantum kit contents</b><br />Components are color coded for ease of use.

技术参数

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MCP-1 Human ProQuantum Immunoassay Kit,MCP-1 Human ProQuantum Immunoassay Kit/人MCP-1 ProQuantum免疫测定试剂盒

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