IL-12 p70 Human Matched Antibody Pair,IL-12 p70 Human Matched Antibody Pair/人IL-12 p70匹配抗体对

2024-10-23

IL-12 p70 Human Matched Antibody Pair/人IL-12 p70匹配抗体对

货号:CHC1563

规格:10 x 96 tests

价格:6963

产品类型:标准品和抗体对

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

<31.2 pg/mL

检测范围31.2-2000 pg/mL

样本类型/体积

血清 100 μL

血浆 100 μL

上清液 100 μL

操作时间

1 hr 30 min

测试时间

24 hr

Homogenous (no wash)

No

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

10 x 96 tests

组份

- 捕获(包被)抗体

- 检测抗体

- 标准品

- 链霉亲和素-HRP

储存温度

2-8℃

Protein nameIL-12 p70
Protein aliasesCLMF p35, CLMF p40, cytotoxic lymphocyte maturation factor 1, p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p35, IL-12 subunit p40, IL-12, subunit p35, IL-12A, IL-12B, IL12, subunit p40, IL35 subunit, interleukin 12, p40, interleukin 12A (natural killer cell stimulatory factor 1, p35), interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40), interleukin-12 alpha chain, interleukin-12 beta chain, Interleukin-12 subunit alpha, Interleukin-12 subunit beta, natural killer cell stimulatory factor, 40 kD subunit, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NK cell stimulatory factor chain 2, NKSF1, NKSF2

物种(Tested)

试剂盒类型

匹配抗体对

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesCLMF, CLMF2, IL-12A, IL-12B, IL12A, IL12B, IMD28, IMD29, NFSK, NKSF, NKSF1, NKSF2, P35
Gene ID(Human) 3592,(Human) 3593
Gene symbolIL12A, IL12B
UniProt ID(Human) P29459,(Human) P29460
The Human Interleukin 12 p70 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-12 p70. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate.Principle of the methodELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.Target informationInterleukin-12 (IL-12) is a heterodimeric 70 kDa cytokine composed of two covalently linked, glycosylated chains with molecular weights of 40kD (p40) and 35-kD (p35). IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-g) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. Studies have established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-g and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation. Recombinant mouse IL-12 p70 is produced in baculovirus-infected insect cells as an authentic heterodimer of precursor p35 and p40 subunits using a dual promoter expression system. IL-12 p70 is distinct from other available forms of the protein in that it is expressed as a true heterodimer, as opposed to a single-chain, pseudo-heterodimer in which the subunits are joined by an artificial linker. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. Human and mouse IL-12 p40 share about 70% amino acid sequence homology.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据:

IL-12 p70 Human Matched Antibody PairRepresentative standard curve for Human IL-12 p70 ELISA
技术参数
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