IL-1 beta Human Matched Antibody Pair,IL-1 beta Human Matched Antibody Pair/人IL-1 β匹配抗体对-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

IL-1 beta Human Matched Antibody Pair,IL-1 beta Human Matched Antibody Pair/人IL-1 β匹配抗体对

2024-10-23

IL-1 beta Human Matched Antibody Pair/人IL-1 β匹配抗体对

货号：CHC1213

规格：10 x 96 tests

价格：6931

产品类型：标准品和抗体对

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	<31.2 pg/mL
检测范围	31.2-2000 pg/mL
样本类型/体积	血清 100 μL 血浆 100 μL 上清液 100 μL
操作时间	1 hr 30 min
测试时间	24 hr
Homogenous (no wash)	No
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 捕获（包被）抗体 - 检测抗体 - 标准品 - 链霉亲和素-HRP
储存温度	2-8℃
Protein name	IL-1 beta
Protein aliases	Catabolin, IL-1 beta, interleukin 1, beta, Interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta
物种（Tested）	人
试剂盒类型	匹配抗体对
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	IL-1, IL1-BETA, IL1B, IL1F2
Gene ID	(Human) 3553
Gene symbol	IL1B
UniProt ID	(Human) P01584

The Human IL-1β Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-1β. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate.Principle of the methodELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.Target informationInterleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据：

IL-1 beta Human Matched Antibody PairRepresentative standard curve was generated by following the recommended assay procedure, which includes the use of the Invitrogen CytoSet™ Buffer Set (<a href="https://www.thermofisher.com/order/catalog/product/CNB0011">Product # CNB0011</a>)

技术参数

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IL-1 beta Human Matched Antibody Pair,IL-1 beta Human Matched Antibody Pair/人IL-1 β匹配抗体对

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