IL-10 Human Matched Antibody Pair,IL-10 Human Matched Antibody Pair/人IL-10匹配抗体对-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

IL-10 Human Matched Antibody Pair,IL-10 Human Matched Antibody Pair/人IL-10匹配抗体对

2024-10-23

IL-10 Human Matched Antibody Pair/人IL-10匹配抗体对

货号：CHC1323

规格：10 x 96 tests

价格：6948

产品类型：标准品和抗体对

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	31.2 pg/mL
检测范围	31.2-2000 pg/mL
样本类型/体积	血清 100 μL 血浆 100 μL 上清液 100 μL
操作时间	1 hr 30 min
测试时间	24 hr
Homogenous (no wash)	No
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 捕获（包被）抗体 - 检测抗体 - 标准品 - 链霉亲和素-HRP
储存温度	2-8℃
Protein name	IL-10
Protein aliases	CSIF, Cytokine synthesis inhibitory factor, IL-10, Interleukin-10, T-cell growth inhibitory factor
物种（Tested）	人
试剂盒类型	匹配抗体对
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	CSIF, GVHDS, IL-10, IL10, IL10A, TGIF
Gene ID	(Human) 3586
Gene symbol	IL10
UniProt ID	(Human) P22301

The Human IL-10 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-10. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate.Principle of the methodELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.Target informationInterleukin 10 (IL-10, CSIF) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 is an 18.6 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein (BCRFI). The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells, enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells. Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). IL-10 inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Knockout studies in mice suggest the function of this cytokine as an essential immunoregulator in the intestinal tract.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据：

IL-10 Human Matched Antibody PairMouse IL-10 CytoSet (Product # CMC0104). Lot-to-lot consistency graph, showing 5 different lots of IL-10 CytoSet tested over a 4-year period.

技术参数

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IL-10 Human Matched Antibody Pair,IL-10 Human Matched Antibody Pair/人IL-10匹配抗体对

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