MMP3 Human Matched Antibody Pair,MMP3 Human Matched Antibody Pair/人MMP3匹配抗体对

2024-10-23

MMP3 Human Matched Antibody Pair/人MMP3匹配抗体对

货号:CHC1543

规格:10 x 96 tests

价格:6915

产品类型:标准品和抗体对

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

<1.25 ng/mL

检测范围1.25-20 ng/mL

样本类型/体积

血清 100 μL

血浆 100 μL

上清液 100 μL

操作时间

1 hr 30 min

测试时间

24 hr

Homogenous (no wash)

No

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

10 x 96 tests

组份

- 捕获(包被)抗体

- 检测抗体

- 标准品

- 链霉亲和素-HRP

储存温度

2-8℃

Protein nameMMP3
Protein aliasesmatrix metalloproteinase 3 (stromelysin 1, progelatinase), Matrix metalloproteinase-3, MMP-3, proteoglycanase, SL-1, Transin-1, Stromelysin-1

物种(Tested)

试剂盒类型

匹配抗体对

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesCHDS6, MMP-3, MMP3, SL-1, STMY, STMY1, STR1
Gene ID(Human) 4314
Gene symbolMMP3
UniProt ID(Human) P08254
The Human MMP3 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human MMP3. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate.Principle of the methodELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.Target informationMMP3 (stromelysin-1) belongs to the peptidase M10A subfamily. It is secreted in its pro-protein form, and is activated by extracellular proteinase cleavage. MMP3 is capable of binding to zinc and calcium ions, and has metallo-endopeptidase activity. The active enzyme degrades collagen, gelatin, fibronectin, and laminin. It is involved in normal cellular processes such as wound repair and tissue remodeling. Mutations in the gene can result in coronary heart disease 6.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据:

MMP3 Human Matched Antibody PairRepresentative standard curve for Human MMP3 ELISA
技术参数
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