TGF beta Human Matched Antibody Pair,TGF beta Human Matched Antibody Pair/人TGF β匹配抗体对-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

TGF beta Human Matched Antibody Pair,TGF beta Human Matched Antibody Pair/人TGF β匹配抗体对

2024-10-23

TGF beta Human Matched Antibody Pair/人TGF β匹配抗体对

货号：CHC1683

规格：10 x 96 tests

价格：7048

产品类型：标准品和抗体对

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	<62.5 pg/mL
检测范围	62.5-2000 pg/mL
样本类型/体积	血清 100 μL 血浆 100 μL 上清液 100 μL
操作时间	1 hr 30 min
测试时间	24 hr
Homogenous (no wash)	No
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 捕获（包被）抗体 - 检测抗体 - 标准品 - 链霉亲和素-HRP
储存温度	2-8℃
Protein name	TGF beta-1
Protein aliases	LAP, Latency-associated peptide, prepro-transforming growth factor beta-1, TGF-beta-1, Transforming growth factor beta-1, Transforming growth factor beta-1 proprotein
物种（Tested）	人
试剂盒类型	匹配抗体对
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	CED, DPD1, LAP, TGFB, TGFB1, TGFbeta
Gene ID	(Human) 7040
Gene symbol	TGFB1
UniProt ID	(Human) P01137

The Human TGFβ Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human TGFβ. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate.Principle of the methodELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.Target informationTGF beta-1 (TGFB1, Transforming Growth Factor Beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its pro-domain. Activation of TGF beta-1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF beta-1, an amino-terminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP. TGF beta-1 helps regulates proliferation, differentiation, adhesion, migration in many cell types. Many cells have TGF beta receptors, and the protein positively and negatively regulates many other growth factors. TGF beta-1 is cleaved into a latency-associated peptide and a mature TGF beta-1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGF beta-1 homodimer. The mature peptide may also form heterodimers with other TGF beta family members. The gene for TGF beta-1 is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease and cystic fibrosis.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据：

TGF beta Human Matched Antibody PairMulti-species TGF-beta1 CytoSet™. Representative standard curve generated using matched antibody pair. This CytoSet™ is suitable for quantification of human, mouse, rat and swine samples.

技术参数

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TGF beta Human Matched Antibody Pair,TGF beta Human Matched Antibody Pair/人TGF β匹配抗体对

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