ICAM-1 (Soluble) Human Matched Antibody Pair/人ICAM-1（可溶）匹配抗体对
货号：BMS201MST
规格：10 x 96 tests
价格：6614
产品类型：标准品和抗体对
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 6.25 ng/mL |
| 检测范围 | 6.25-100 ng/mL |
样本类型/体积 | 血清 10 μL 血浆 10 μL 上清液 10 μL |
操作时间 | 1 hr 10 min |
测试时间 | 24 hr |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 10 x 96 tests |
组份 | - 捕获（包被）抗体 - 检测抗体 - 标准品 - 样品稀释液 |
储存温度 | -20°C |
| Protein name | ICAM-1 (CD54) |
| Protein aliases | CD54, cell surface glycoprotein P3.58, ICAM-1, IL-1 beta, Catabolin, Intercellular adhesion molecule 1, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, Major group rhinovirus receptor |
物种（Tested） | 人 |
试剂盒类型 | 匹配抗体对 |
标记（染料） | HRP |
| 检测抗体偶联物 | HRP |
| Gene aliases | BB2, CD54, ICAM1, P3.58 |
| Gene ID | (Human) 3383 |
| Gene symbol | ICAM1 |
| UniProt ID | (Human) P05362 |
The Human ICAM-1 Matched Antibody Pair Kit comes with pre-matched antibody pairs, standards, and sample diluent to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human ICAM-1. Buffer reagents and microplates needed to complete the ELISA reaction are sold separately.Principle of the methodELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.Target informationICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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