IL-12/IL-23 p40 Human Matched Antibody Pair/人IL-12/IL-23 p40匹配抗体对

货号：BMS2013MST

规格：10 x 96 tests

价格：8678

产品类型：标准品和抗体对

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	31 pg/mL
检测范围	31-2000 pg/mL
样本类型/体积	血清 50 μL 血浆 50 μL 上清液 50 μL
操作时间	4 hr 10 min
测试时间	24 hr
Homogenous (no wash)	No
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 捕获（包被）抗体 - 检测抗体 - 标准品 - SAV-HRP - 样品稀释液
储存温度	2-8°C
Protein name	IL-12/IL-23 p40
Protein aliases	CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, IL-12B, IL-23 subunit alpha, IL-23-A, IL-23p19, IL12, subunit p40, interleukin 12, p40, interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40), interleukin 23 p19 subunit, interleukin 23, alpha subunit p19, interleukin-12 beta chain, Interleukin-12 subunit beta, Interleukin-23 subunit alpha, Interleukin-23 subunit p19, interleukin-six, G-CSF related factor, JKA3 induced upon T-cell activation, natural killer cell stimulatory factor, 40 kD subunit, NK cell stimulatory factor chain 2, NKSF2
物种（Tested）	人
试剂盒类型	匹配抗体对
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	CLMF, CLMF2, IL-12B, IL-23, IL-23A, IL12B, IL23A, IL23P19, IMD28, IMD29, NKSF, NKSF2, P19, SGRF, UNQ2498/PRO5798
Gene ID	(Human) 3593,(Human) 51561
Gene symbol	IL12B, IL23A
UniProt ID	(Human) P29460,(Human) Q9NPF7

The Human IL-12/23 p40 Matched Antibody Pair Kit comes with pre-matched antibody pairs, standards, sample diluent and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-12/23 p40. Buffer reagents and microplates needed to complete the ELISA reaction are sold separately.Principle of the methodELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.Target informationIL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

技术参数

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