TNF alpha Human Matched Antibody Pair/人TNF α匹配抗体对

货号：BMS2034MST

规格：10 x 96 tests

价格：6614

产品类型：标准品和抗体对

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	23.4 pg/mL
检测范围	23.4-1500 pg/mL
样本类型/体积	血清 50 μL 血浆 50 μL 上清液 50 μL
操作时间	1 hr 10 min
测试时间	24 hr
Homogenous (no wash)	No
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 捕获（包被）抗体 - 检测抗体 - 标准品 - SAV-HRP偶联 - 样品稀释液
储存温度	-20°C
Protein name	TNF alpha
Protein aliases	APC1 protein, C-domain 1, C-domain 2, Cachectin, ICD1, ICD2, Intracellular domain 1, Intracellular domain 2, N-terminal fragment, NTF, TNF, macrophage-derived, monocyte-derived, TNF-a, TNF-alpha, Tumor necrosis factor, tumor necrosis factor ligand 1F, Tumor necrosis factor ligand superfamily member 2, membrane form, soluble form, tumor necrosis factor-alpha
物种（Tested）	人
试剂盒类型	匹配抗体对
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	DIF, TNF, TNF-alpha, TNFA, TNFSF2, TNLG1F
Gene ID	(Human) 7124
Gene symbol	TNF
UniProt ID	(Human) P01375

The Human TNF-alpha Matched Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human TNF-alpha. Buffer reagents and microplates needed to complete the ELISA reaction are sold separately.Principle of the methodELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.Target informationTNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

技术参数

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