Cytochrome C Human Matched Antibody Pair/人细胞色素C匹配抗体对

货号：BMS263MST

规格：10 x 96 tests

价格：6614

产品类型：标准品和抗体对

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	0.08 ng/mL
检测范围	0.08-5 ng/mL
样本类型/体积	血清 50 μL 血浆 10 μL 细胞裂解液 2 μL
操作时间	3 hr 10 min
测试时间	24 hr
Homogenous (no wash)	No
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 捕获（包被）抗体 - 检测抗体 - 标准品 - SAV-HRP - 分析液 - 裂解液
储存温度	-20°C
Protein name	Cytochrome C
Protein aliases	Cytochrome c
物种（Tested）	人
试剂盒类型	匹配抗体对
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	CYC, CYCS, HCS, THC4
Gene ID	(Human) 54205
Gene symbol	CYCS
UniProt ID	(Human) P99999

The Human Cytochrome C Matched Antibody Pair Kit comes with pre-matched antibody pairs, standards, assay buffer, lysis buffer and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human Cytochrome C. Buffer reagents and microplates needed to complete the ELISA reaction are sold separately.Principle of the methodELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.Target informationCytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

技术参数

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