Amyloid beta 40 Mouse ELISA Kit ELISA试剂盒,Amyloid beta 40 Mouse ELISA Kit/小鼠淀粉样蛋白β 40 ELISA试剂盒

2024-10-23

Amyloid beta 40 Mouse ELISA Kit/小鼠淀粉样蛋白β 40 ELISA试剂盒

货号:KMB3481

规格:96 tests

价格:10646

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:小鼠

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

<5 pg/mL

检测范围7.81-500 pg/mL

样本类型/体积

脑脊液 10 µL

上清液 10 µL

组织匀浆 10 µL

操作时间

1 hr 20 min

测试时间

4 hr

批间精密度(CV)4.8%
批内精密度(CV)5%

Homogenous (no wash)

No

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

96 tests

组份

- 预包被96孔板

- 标准品

- 标准稀释液

- 生物素化检测抗体

- 链霉亲和素-HRP

- HRP稀释液

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8°C

Protein nameAmyloid beta 40
Protein aliasesAbeta40, Abeta42, ABPP, AG, AID(50), AID(57), AID(59), Alpha-CTF, Alpha-secretase C-terminal fragment, Alzheimer disease amyloid A4 protein homolog, amyloid beta A4 protein, Amyloid intracellular domain 50, Amyloid intracellular domain 57, Amyloid intracellular domain 59, Amyloid precursor protein, Amyloid-beta A4 protein, Amyloid-beta precursor protein, Amyloid-beta protein 40, Amyloid-beta protein 42, Amyloidogenic glycoprotein, APP, APP-C57, APP-C59, APP-C99, appican, Beta-APP40, Beta-APP42, Beta-CTF, Beta-secretase C-terminal fragment, C31, C80, C83, C99, Gamma-CTF(50), Gamma-CTF(57), Gamma-CTF(59), Gamma-secretase C-terminal fragment 50, Gamma-secretase C-terminal fragment 57, Gamma-secretase C-terminal fragment 59, N-APP, P3(40), P3(42), protease nexin II, S-APP-alpha, S-APP-beta, Soluble APP-alpha, Soluble APP-beta

物种(Tested)

小鼠

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesAbeta, Abpp, Adap, Ag, App, betaApp, Cvap, E030013M08Rik
Gene ID(Mouse) 11820
Gene symbolApp
UniProt ID(Mouse) P12023
The Mouse Amyloid Beta 42 (Ms Abeta 42) ELISA quantitates Ms Abeta 42 in human cerebrospinal fluid, tissue homogenates and culture supernatant. The assay will exclusively recognize both natural and recombinant Ms Abeta 42.小鼠淀粉样β 42(小鼠 β 42)ELISA定量检测人脑脊髓液,组织匀浆和培养上清液中的小鼠β 42。 该检测试剂盒将专门识别天然和重组小鼠β 42。Principle of the method 方法原理The Mouse Abeta 42 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠β 42固相夹心ELISA(酶联免疫吸附测定)用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息Beta-amyloid is a 40–43 amino acid peptide cleaved from amyloid precursor protein, and a putative gamma-secretase. Amyloid beta (1-40) together with Amyloid beta (1-42) are two major C-terminal variants of the amyloid beta protein, constituting the majority of amyloid beta proteins. Both the amyloid beta (40) and amyloid beta (42) alloforms are major components of the extracellular plaques found in Alzheimer's disease brain tissue.β-淀粉样蛋白是从淀粉样蛋白前体蛋白和推定的γ-分泌酶切下的40-43个氨基酸肽。 淀粉样蛋白β(1-40)与淀粉样蛋白β(1-42)一起是淀粉样蛋白β蛋白的两个主要C端变异体,构成了大多数淀粉样蛋白β蛋白。 淀粉样蛋白β(40)和淀粉样蛋白β(42)异形体都是在阿尔茨海默氏病脑组织中发现的细胞外斑块的主要成分。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

Amyloid beta 40 Mouse ELISA KitRepresentative standard curve for Mouse Beta Amyloid 1-40 ELISA, with parallel titrations of natural Mouse Abeta 1-40 in Neuro2a cell culture supernatant.

技术参数
© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价,请加客服QQ:1749072012 、客服微信:jinshanbio,或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务,数据资源基于CC协议。
© 本文地址:https://gibcohyclone.cn/thread-45130.htm
产品询价需求提交
返回
扫码添加客服微信,咨询产品报价