IFN gamma Human Uncoated ELISA Kit/人IFN γ未包被ELISA试剂盒

货号：ESS0002

规格：5 x 96 tests

价格：7267

产品类型：自定义ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	<15.6 pg/mL
检测范围	15.6-1000 pg/mL
样本类型/体积	上清液 100 μL 血清 100 μL
操作时间	24 hr
测试时间	2 hr 50 min
Homogenous (no wash)	No
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	5 x 96 tests
组份	- 包被抗体 - 冻干重组标准 - 检测抗体 - 链霉亲和素-HRP - 底物溶液 - 即用型终止液，br> 0.16M硫酸 - 分析证书：特定批次的抗体和标准品稀释说明
储存温度	2-8℃
Protein name	IFN gamma
Protein aliases	IFN-gamma, Immune interferon, Interferon gamma, interferon, gamma
物种（Tested）	人
试剂盒类型	Uncoated ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	IFG, IFI, IFNG
Gene ID	(Human) 3458
Gene symbol	IFNG
UniProt ID	(Human) P01579

The Human Interferon gamma Uncoated ELISA Kit contains pre-matched antibody pairs and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IFN-gamma.人干扰素γ未包被的ELISA试剂盒包含预先匹配的抗体对和试剂，用于执行定量酶联免疫吸附测定（ELISA），以检测和定量人IFN-γ的蛋白质水平。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA用于测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被在微孔板孔的底部，这是一个过夜的过程。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.IFNγ（干扰素γ，II型干扰素）是一种巨噬细胞激活因子，是免疫干扰素，主要由T淋巴细胞和自然杀伤细胞响应抗原，促细胞分裂剂，葡萄球菌肠毒素B，植物血凝素和其他细胞因子而产生。 IFNγ是由两个146个氨基酸亚基组成的二聚体蛋白。 IFNγ是一种糖蛋白，功能上以约45 kDa的同型二聚体形式存在。在SDS-PAGE上，由于差异糖基化，IFNγ出现为25、20和较小的15.5 kDa条带的组合。 IFNγ同二聚体的生物活性是高度物种特异性的。人IFNγ与小鼠无交叉反应。 IFNγ功能包括：抗病毒活性，肿瘤抗增殖活性，I类和II类MHC的诱导，巨噬细胞活化以及B淋巴细胞增强的免疫球蛋白分泌。 IFNγ参与细胞因子的调节，并与其他细胞因子协同作用。 IFNγ的激活是通过结合IFNγI和II并激活JAK-STAT途径进行的。 IFNγ与IFNα或IFNβ没有任何同源性，但是人IFNγ与小鼠IFNγ具有约40％的序列同源性。 IFNγ被IL2，FGF碱性蛋白，EGF上调，而被维生素D3或DMN下调。 IFNγ基因突变与再生障碍性贫血有关。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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