CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), eBioscience/CD223（LAG-3）单克隆抗体（eBioC9B7W（C9B7W））

货号：14-2231-81,14-2231-82,14-2231-85

规格：50 µg ,100 µg,500 µg

价格：1621,1820,3649

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：其它

克隆号：eBioC9B7W (C9B7W)

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	0.5 µg/test（Flow）；2 µg/mL（ELISA)；Assay-Dependent(FN)；Assay-Dependent(IP)

产品详细信息Description: The eBioC9B7W monoclonal antibody recognizes mouse CD223 (LAG-3, LAG3) protein expressed by activated alpha/beta-TCR bearing T cells, a subset of gamma/delta-TCR bearing T cells and a subset of NK cells. CD223 is a 70 kDa type I transmembrane protein with a structure that is similar to CD4. However, a soluble form of human CD223 has been detected by ELISA in human serum, and data suggest that mouse CD223 is proteolytically cleaved in the D4 domain. This results in a 54 kDa fragmentcontaining all the extracellular domains, and a 16 kDa fragment containing the intracellular and transmembrane domains. The 54 kDa can remain membrane-associated or be released as soluble CD223.CD223 binds to MHC class II with higher affinity than CD4, and it is thought that this interaction is involved in the negative regulation of T-cell activation and homeostatic proliferation. Furthermore, CD223 is expressed by CD4+CD25+ regulatory T cells, and it has been suggested that CD223 may be involved in their regulatory function.Applications Reported: This eBioC9B7W (C9B7W) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and ELISA. (Please use Functional Grade purified eBioC9B7W (C9B7W), cat. 16-2231, in functional assays.).Applications Tested: This eBioC9B7W (C9B7W) antibody has been tested by flow cytometric analysis of anti-CD3 and anti-CD28-activated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.This eBioC9B7W (C9B7W) antibody has also been tested by ELISA analysis of supernatant produced from mouse CD223-transfected 3A9 cells. This can be used in ELISA at 2 µg/mL for capture of the secreted form of CD223, together with the polyclonal anti-mouse CD223 (cat. 24-2232) as the detection antibody.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).

数据

CD223 (LAG-3) Antibody (14-2231-81) in FlowStaining of 3-day Anti-Mouse CD3 (Product # 16-0031-82) and Anti-Mouse CD28 (Product # 16-0281-82)-stimulated C57BL/6 splenocytes with 0.25 µg of Rat IgG1 K Isotype Control Purified (Product # 14-4301-82) (open histogram) or 0.25 µg of Anti-Mouse CD223 (Lag-3) Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis. CD223 (LAG-3) Antibody (14-2231-81)Cell 2016 -Figure 5 Villin-Specific Btnl1 Induction Rescues Vgamma7 + IEL In Vivo (A-D) Week 7-13 (adult) or day 7-21 (pups) mice of indicated genotypes on a Btnl1 -/- background were administered Dox (1 mg/ml, 2% sucrose) or control water (2% sucrose) for times indicated, and IELs were analyzed by flow cytometry. n >= 5 (A and B); n >= 6 (C and D). (E) Comparative cell-surface phenotypes of Vgamma7 + IELs from week 4-5 WT mice and animals indicated (n = 4-8). (A) is representative of two or more independent experiments; (B)-(E) present data pooled from three or more experiments. Statistical significance in (C) was determined using the Holm-Sidak method. All error bars represent mean +- SD. See also Figure S5 . Figure 5 CD223 (LAG-3) Antibody (14-2231-81)Cell 2016 -Figure S1 Phenotypic Differences between CD122 HI Vgamma7 + IELs and Other IEL Subsets, Related to Figure 1 (A) Cell surface phenotype of Vgamma7 + , Vgamma7 - (CD3 + TCRbeta - Vgamma7 - ) and alphabeta (TCRbeta + ) IEL from 3-5 week old (W3-5) C57Bl/6 (WT) mice (n >= 7). (B) Cell surface phenotype of WT Vgamma7 + CD122 HI versus Vgamma7 + CD122 LO IEL (n >= 7). (C) Heat map of genes differentially expressed (log-2-FoldChange) between Vgamma7 + CD122 hi and Vgamma7 + CD122 lo IEL sorted from D14-D17 WT mice. Data generated by RNA sequencing ( and apos;cell cycle and apos; and and apos;cell surface and apos; GO terms annotated). Values scaled to their median value across the samples. (D) 3hr EdU incorporation in vivo in Vgamma7 + versus Vgamma7 - IEL from D28 WT mice assessed by flow cytometry in indicated IEL subsets (Vgamma7 - are CD3 + TCRbeta - Vgamma7 - ). Data are representative of 1 (C) or >= 3 (A,B) independent experiments. Panel (D) presents data pooled from 3 independent experiments. All error bars represent mean +- SD. Related to Figure 1 . Figure S1 CD223 (LAG-3) Antibody (14-2231-81)Journal of immunology (Baltimore, Md. : 1950) 2016 -Published figure using CD223 (LAG-3) monoclonal antibody (Product # 14-2231-81)

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参考文献：

1.Scientific reportsCharacterization of c-Maf+Foxp3-Regulatory T Cells Induced by Repeated Stimulation of Antigen-Presenting B Cells.2.Nature communicationsRational combination of oncolytic vaccinia virus and PD-L1 blockade works synergistically to enhance therapeutic efficacy.3.CellEpithelia Use Butyrophilin-like Molecules to Shape Organ-Specific γδ T Cell Compartments.4.Journal of immunology (Baltimore, Md. : 1950)Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency.5.Journal for immunotherapy of cancerCombined immune checkpoint protein blockade and low dose whole body irradiation as immunotherapy for myeloma.

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