Mouse anti Human 14-3-3 epsilon antibody BIO-RAD VMA00525 Mouse anti Human 14-3-3 epsilon antibody

2024-10-28

Mouse anti Human 14-3-3 epsilon antibody

货号:VMA00525

规格:100μl

市场价格:2916

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:14-3-3 epsilon

物种:人

宿主:小鼠

抗体亚型:IgG2a

克隆号:AB01/1G9

荧光染料:Purified

产品介绍
Mouse anti Human 14-3-3 epsilon antibodyrecognizes the 14-3-3 protein epsilon, also known as epididymis luminal protein 2, mitochondrial import stimulation factor L subunit, protein kinase C inhibitor protein-1, or tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide, or YWHAE.YWHAE gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for YWHAE (provided by RefSeq, Aug 2008).Mouse anti Human 14-3-3 epsilon antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
产品详情
Target SpeciesHuman
Product FormPurified IgG - liquid
PreparationMouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3).
ImmunogenE. coli-derived recombinant human 14-3-3 epsilon (amino acids 1 - 255)
AcknowledgementsPrecisionAb is a trademark of Bio-Rad Laboratories.
RegulatoryFor research purposes only
Guarantee12 months from date of despatch.
存储条件
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
应用
Application NameVerifiedMin DilutionMax Dilution
Western Blotting1/1000
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood.
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技术参数

产品应用 wb

产品类型 单克隆抗体

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