IL-2 Monoclonal Antibody (JES6-1A12), eBioscience/IL-2单克隆抗体（JES6-1A12）

货号：14-7022-81,14-7022-85

规格：50 µg,500 µg

价格：1175,3363

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：JES6-1A12

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	Flow；2-6 µg/mL（ELISA)

产品详细信息Description: The JES6-1A12 antibody reacts with mouse interleukin-2 (IL-2). The JES6-1A12 antibody is a neutralizing antibody. Mouse IL-2 (also known as TCGF) is a 17 kDa factor produced mainly by activated CD4+ T cells. IL-2 induces cell cycle progression of resting cells in an antigen non-specific manner and allows clonal expansion of activated T cells. IL-2 also acts on activated B cells, monocytes, NK cells, LAK cells, and on oligodendroglial cells in vitro. In addition, IL-2 plays a role inhematopoiesis, tumor surveillance and anti-inflammatory reactions, and hence is a central regulator of the immune response. Non-glycosylated IL-2 is biologically active.Applications Reported: The JES6-1A12 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization of IL-2 bioactivity, IP, and intracellular staining.Applications Tested: The JES6-1A12 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse Interleukin-2 (IL-2) in combination with the biotin JES6-5H4 (13-7021) antibody for detection and recombinant mouse IL-2 (14-8021) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

数据

IL-2 Antibody (14-7022-81) in ELISASplenocytes from mice pre-immunized with PLP139-151 peptide were recall activated 4 weeks later with PLP139-151 for 24 hours in Mouse IL-2 ELISPOT assay. Control well is medium alone. IL-2 Antibody (14-7022-81)Nature communications 2017 -Figure 3 CIC negatively regulates T-cell activation. ( a , b ) Increased CIC expression in activated CD4 + T cells. Western blot analysis for CIC levels in CD4 + T cells during T-cell activation by alpha-CD3/CD28 antibodies ( a ) and in sorted naive (CD44 lo CD62L hi ) and effector/memory (CD44 hi CD62L lo ) CD4 + T cells ( b ). ( c ) ELISA of IL-2. WT and Cic -deficient (cKO) CD4 + CD25 - CD44 lo CD62L hi naive T cells were stimulated with plate-bound anti-CD3 (1.0 mug ml -1 ) in the presence (right) or absence (left) of plate-bound anti-CD28 (2.0 mug ml -1 ). The supernatants were taken 48 h and 72 h after stimulation and subjected to ELISA for IL-2 concentration. n =4 per each sample. Error bars indicate s.e.m. * P <0.05, ** P <0.01 and *** P <0.001 (two-tailed two-sample unequal variance Student t -test). ( d ) In vitro T-cell proliferation assay. Naive CD4 + T cells purified from pooled spleens and lymph nodes of Cic f/f and Cic f/f Vav1-Cre mice were labelled with CTV dye and stimulated with plate-bound anti-CD3 (1.0 mug ml -1 ) in the presence (right) or absence (left) of plate-bound anti-CD28 (2.0 mug ml -1 ). The cells were analysed 72 h after stimulation. Data are representative of three independent experiments. Shaded area: unstimulated control, blue line: Cic f/f , red line: Cic f/f Vav1-Cre . IL-2 Antibody (14-7022-81)PLoS pathogens 2014 -Figure 2 High fecal bacterial load is associated with dampened splenic T cell response. A-E: Twelve mice are represented and data is representative of 3 independent experiments with a total of 30 mice. Each circle represents an individual mouse. Asterisks indicate significant R values determined using Spearman and apos;s correlation, two-tailed. C-F: Each point represents a single mouse with red and orange dots representing super-shedders as confirmed by cecal and colonic inflammation. A,B: Tbet + (T H 1) and FoxP3 + (T regs ) CD4 T cells were measured as a percentage of total CD4 T cells in the spleen. Neutrophils were measured as a percentage of total splenocytes. Splenic T H 1 and T regs were significantly negatively correlated with a R value of -0.58*. Fecal bacterial load was negatively correlated with splenic T H 1 (R = -0.49) and significantly positively correlated with splenic T regs (R = +0.65*). Finally, splenic neutrophils were significantly positively correlated with fecal bacterial load (R = +0.9*) and negatively correlated with T H 1 cells (R = -0.43). C: Splenocytes were stimulated ex vivo for 15 minutes with 40 ng/ml IL-2. IL-2 responsive pSTAT5 + CD4 T cells were gated and the mean fluorescence intensity (MFI) of pSTAT5 measured. D: Ki-67 + cells were quantified as a percentage of total CD4 T cells and plotted against fecal bacterial burden of the mice. E : Splenocytes were stimulated ex vivo for 15 minutes with 40 ng/ml IL-6.

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参考文献：

1.The Journal of experimental medicineRab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation.2.Journal of the American Society of Nephrology : JASNOpposing Roles of Dendritic Cell Subsets in Experimental GN.3.European journal of immunologyToll like Receptor 2 engagement on CD4+T cells promotes TH9 differentiation and function.4. The Journal of experimental medicineCD28-inducible transcription factor DEC1 is required for efficient autoreactive CD4+ T cell response.5. Journal of immunology (Baltimore, Md. : 1950)CD70-driven costimulation induces survival or Fas-mediated apoptosis of T cells depending on antigenic load.

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