IL-4 Monoclonal Antibody (11B11), eBioscience/IL-4单克隆抗体（11B11）

货号：14-7041-81,14-7041-85

规格：50 µg,500 µg

价格：1130,3539

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：其它

克隆号：11B11

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	Flow；1-4 µg/mL（ELISA)；FN；TCA；Neu；IV；ICC

产品详细信息Description: The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kD cytokine. The 11B11 antibody is a neutralizing antibody. Mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surfaceMHC class II and CD23.Applications Reported: The 11B11 antibody has been reported for use in ELISA capture, ELISPOT capture, and neutralization of IL-4 bioactivity.Applications Tested: The 11B11 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse Interleukin-4 (IL-4) in combination with the biotin BVD6-24G2 (cat. 13-7042) antibody for detection and recombinant mouse IL-4 (cat. 14-8041) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

数据

IL-4 Antibody (14-7041-81) in ELISASplenocytes from 8 week old mice pre-immunized with OVA/IFA were recall-activated with OVA antigen for 36 hours in Mouse IL-4 ELISPOT assay. Control well is medium alone. IL-4 Antibody (14-7041-81)Genome medicine 2018 -Fig. 6 IRE1a-XBP1 pathway is required for cytokine expression and secretion in Th2 lymphocyte. Naive T helper cells were cultured following Th2 activation condition in the presence of IRE1a inhibitor 4mu8c for 3 days, rested for 2 days, reactivated by coated plate, and analyzed by flow cytometry to detect intra-cellular cytokines IL4, IL5, and IL13 expression. Representative FACS profiles are displayed in the first two columns. The intra-cellular cytokine expression is compared in column 3, with three to seven independent biological replicates. Fourth column: cell culture supernatants from 4mu8c-treated or DMSO-treated Th2 were analyzed by ELISA to measure the cytokine concentration. FACS gating: lymphocytes > singlets > live cells > cytokines

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参考文献：

1.F1000ResearchMyelin-specific T helper 17 cells promote adult hippocampal neurogenesis through indirect mechanisms.2. Mediators of inflammationInteraction between ANXA1 and GATA-3 in Immunosuppression of CD4+T Cells.3. Evidence-based complementary and alternative medicine : eCAMBaicalin Downregulates RLRs Signaling Pathway to Control Influenza A Virus Infection and Improve the Prognosis.4. Biomolecules and therapeuticsSuppressive Effect of 4-Hydroxy-2-(4-Hydroxyphenethyl) Isoindoline-1,3-Dione on Ovalbumin-Induced Allergic Asthma.5. Cancer researchIn VitroPriming of Adoptively Transferred T Cells with a RORγ Agonist Confers Durable Memory and StemnessIn Vivo.

技术参数

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