IL-4 Monoclonal Antibody (8D4-8), eBioscience/IL-4单克隆抗体（8D4-8）

货号：14-7049-81,14-7049-85

规格：50 µg,500 µg

价格：1130,3249

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：其它

克隆号：8D4-8

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	WB；Flow；1-4 µg/mL（ELISA)

产品详细信息Description: The 8D4-8 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells.Applications Reported: The 8D4-8 antibody has been reported for use in capture of human IL-4 by ELISA and in intracellular staining for flow cytometric analysis.Applications Tested: The 8D4-8 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human Interleukin-4 (IL-4) in combination with the biotinylated MP4-25D2 (13-7048) antibodyfor detection and recombinant human IL-4 (14-8049) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate.The fluorochrome-conjugated 8D4-8 antibody is offered in 2 formats:- µg size: has been tested by intracellular staining for flow cytometric analysis. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

数据

IL-4 Antibody (14-7049-81)Nucleic acids research 2015 -Figure 2. piR30840 significantly decreases IL-4 expression. ( A and B ) s30840 decreases IL-4 expression in purified CD4 T lymphocytes from PBMCs. IL-4 expression was detected by qRT-PCR (left panel), northern blotting (right panel) (A) and western blotting (B). The data represents three independent experiments. Con represents the synthesized random sequence for control. s30840 represents the synthesized piR30840. P < 0.05. ( C ) s30840 decreases IL-4 expression in activated CD4 T lymphocytes. IL-4 expression was detected by qRT-PCR (upper panel) and western blotting (lower panel) analyses. The data represents three independent trials. P < 0.05. ( D ) Antisense inhibitor piR30840 antisense upregulates IL-4 in purified CD4 T lymphocytes from PBMCs. IL-4 expression was detected by qRT-PCR (left panel) and western blot (right panel) analyses. The data represents three independent trials. P < 0.05. ( E and F ) The construct containing all the IL-4 exons and introns (construct pIL4) was co-transfected with s30840 into 293T cells. IL-4 expression was detected by qRT-PCR (left panel) and western blotting (right panel) (E), as well as by northern blotting (F) analyses. The data represents three independent experiments. P < 0.01. IL-4 Antibody (14-7049-81)Nucleic acids research 2015 -Figure 6. piR30840 regulates the development of Th2 lymphocytes. ( A and B ) Human CD4 naive cells were cultured in a conditioned medium for Th2 development and then transfected with an s30840 (A) or piR30840 inhibitor (B). IL-4 expression was detected by qRT-PCR and western blotting analyses. P < 0.01. The data represents three independent experiments. ( C ) Detection of IL-4 expression in human PBMCs isolated from humanized NOG mice. IL-4 expression was detected by qRT-PCR analysis. The data was expressed as the mean of three independent experiments. Wilcoxon test was used to calculate the P -value. P < 0.05. ( D ) CD4 naive cells were cultured in the conditioned medium for Th2 development and transfected with s30840 or control. After 7 days, the Th2 T-lymphocytes (intracellular stained as IL-4 + IFN-gamma - ) were detected with FACS analysis. The data was shown as a mean of six independent experiments. P < 0.01. ( E ) The CD4 naive cells were cultured in a conditioned medium for Th2 development with or without the transfection of inhibitor. We adoptively transferred the naive cells into the irradiated NOG mice which were first humanized. After 7 days, Th2 T-lymphocytes (intracellularly stained as IL-4 + IFNgamma - ) were detected by using FACS analysis. The data were expressed as the mean of six independent experiments. Statistical significance between two samples was determined by using the student and apos;s t -test. P < 0.01.

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参考文献：

1.JCI insightA molecular signature of preclinical rheumatoid arthritis triggered by dysregulated PTPN22.2. Journal of immunology (Baltimore, Md. : 1950)Anticardiac myosin immunity and chronic allograft vasculopathy in heart transplant recipients.3. Nucleic acids researchA SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes.4.PloS onePotential role of IL-17-producing iNKT cells in type 1 diabetes.5. Nucleic acids researchA SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes.

技术参数

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