IL-13 Monoclonal Antibody (eBio13A), eBioscience/IL-13单克隆抗体（eBio13A）

货号：14-7133-81,14-7133-85

规格：50 µg,500 µg

价格：1455,3681

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：其它

克隆号：eBio13A

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	Flow；1-4 µg/mL（ELISA)；Neu；FN

产品详细信息Description: The eBio13A antibody reacts with mouse IL-13. IL-13 is a cytokine produced mainly by Th2 cells, but also by antigen-primed CD8 T cells. IL-13 has a strong involvement in allergic inflammation and parasitic clearing and in cancer models has been shown to have either inhibitory or stimulatory activity depending on the tumor. In humans, IL-13 is found to play a role in isotype switching in B cells. IL-13 is implicating in down modulating macrophage activity, through the reduction of pro-inflammatory cytokines (IL-1, IL-6, IL-8, IL-10, IL-12)Applications Reported: The eBio13A antibody has been reported useful for ELISA and ELISPOT capture, as well as intracellular staining for flow cytometric analysis.Applications Tested: The eBio13A antibody has been tested as the capture antibody in a sandwich ELISA (and ELISPOT) for analysis of mouse IL-13, in combination with the biotinylated eBio1316H antibody for detection (13-7135) and recombinant mouse IL-13 (14-8131) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1.0 - 4.0 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8.0 pg/mL should be included in each ELISA plate.The Functional Grade Purified eBio1316H antibody (16-7135) is recommended for in vitro blocking studies.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.

数据

IL-13 Antibody (14-7133-85)Genome medicine 2018 -Fig. 6 IRE1a-XBP1 pathway is required for cytokine expression and secretion in Th2 lymphocyte. Naive T helper cells were cultured following Th2 activation condition in the presence of IRE1a inhibitor 4mu8c for 3 days, rested for 2 days, reactivated by coated plate, and analyzed by flow cytometry to detect intra-cellular cytokines IL4, IL5, and IL13 expression. Representative FACS profiles are displayed in the first two columns. The intra-cellular cytokine expression is compared in column 3, with three to seven independent biological replicates. Fourth column: cell culture supernatants from 4mu8c-treated or DMSO-treated Th2 were analyzed by ELISA to measure the cytokine concentration. FACS gating: lymphocytes > singlets > live cells > cytokines IL-13 Antibody (14-7133-85)Immunity 2017 -Figure 7 AREG Induces EGFR Phosphorylation at Tyr-992, which Allows for the Interaction between T1/ST2 and EGFR WT (gray) and Areg -/- (purple) mice were infected with H. polygyrus , and on day 14 post infection, mLN were harvested. (A and B) mLN cells were stimulated with rIL-33, anti-CD3, or media, and the IL-13 (A) and p-ERK (B) expression was determined by intra-cellular staining and flow cytometry analysis. (C) MHCII-deficient mice were infected with H. polygyrus and 7 days post-infection received flow cytometry-sorted CD4 + T cells derived from mLN of naive or H. polygyrus -infected WT, Egfr fl/fl xCd4-cre , or Areg -/- mice. Worm burden and egg counts were determined 2 weeks post infection. (D-F) mLN cells were stimulated with rIL-33, rAREG, both, or media only, and EGFR p-Y1068 (D), p-ERK (E), and IL-13 (F) expression was determined by flow cytometry analysis. (G) EGFR phosphorylation at position Y992 on CD4 + T cells derived from mLN of WT or Areg -/- H. polygyrus -infected mice in the presence or absence of rAREG. (H) HEK293T cells were transfected as indicated with T1/ST2 and the IL-1RacP in combination with WT EGFR or EGFR Y992F mutant. Subsequently, the cell lysates were analyzed for the expression of the transfected proteins (input, left panel). The same lysates were also subjected to an EGFR-specific immunoprecipitation (EGFR-IP, right panel) or were treated with the isotype control (iso, right panel). Precipitates were analyzed by immunoblot.

推荐产品：

流式抗体

参考文献：

1.Genome medicineGenome-wide analyses reveal the IRE1a-XBP1 pathway promotes T helper cell differentiation by resolving secretory stress and accelerating proliferation.2. Nature communicationsT cell-intrinsic IL-1R signaling licenses effector cytokine production by memory CD4 T cells.3. ImmunityAutoimmune Th17 Cells Induced Synovial Stromal and Innate Lymphoid Cell Secretion of the Cytokine GM-CSF to Initiate and Augment Autoimmune Arthritis.4. The Journal of allergy and clinical immunologyAllergen-specific IgG antibody signaling through FcγRIIb promotes food tolerance.5. Immunity, inflammation and diseaseIntratracheal myriocin enhances allergen-induced Th2 inflammation and airway hyper-responsiveness.

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-45208.htm