Phospho-S6 (Ser235, Ser236) Monoclonal Antibody (cupk43k)

货号：14-9007-80,14-9007-82

规格：25 μg,100μg

价格：0,3090

产品类型：流式抗体

品牌：eBioscience

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG

克隆号：cupk43k

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	Assay-Dependent(Flow)；1:1000(WB)

产品详细信息Description: This cupk43k monoclonal antibody recognizes human and mouse ribosomal protein S6 (also known as 40S ribosomal protein S6, phosphoprotein NP33, RPS6, RS6, S6) when phosphorylated on serine 235 (S235, human) and serine 236 (S236, mouse). Ribosomal protein S6 is a component of the 40S subunit of the ribosome and is phosphorylated at multiple sites following stimulation of cells by growth factors, tumor promoting agents, or mitogens. Phosphorylation of ribosomal protein S6 by p70S6K andPKDCD results in upregulation of the translation of RNA coding for proteins involved in cell cycle entry. Ribosomal protein S6 is dephosphorylated upon growth arrest.The specificity of the cupk43k monoclonal antibody was determined by western blotting.Applications Reported: This cupk43k antibody has been reported for use in intracellular staining followed by flow cytometric analysis, and western blotting. (Fluorochrome conjugated cupk43k is recommended for use in intracellular staining and flow cytometric analysis.).Applications Tested: This cupk43k antibody has been tested by immunoblotting of stimulated normal human peripheral blood cells. This can be used at less than or equal to 5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated bydifferent protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

数据

Phospho-S6 (Ser235, Ser236) Antibody (14-9007-80) in WBWestern blot was performed using Anti-Phospho-S6 (Ser235, Ser236) Monoclonal Antibody (cupk43k), eBioscience™(Product # 14-9007-82) and a 32 kDa band corresponding to Phospho-S6 (Ser235, Ser236) was observed across the cell lines tested. Whole cell extracts (30 µg lysate) of U-2 OS (Lane 1), U-2 OS treated with IGF (Lane 2), U-2 OS treated with Rapamycin (Lane 3), U-2 OS treated with both IGF and Rapamycin (Lane 4) and HEK-293 (Lane 5) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). The cells were treated with a concentration of 50 ng/mL IGF for 6 hrs and 50 nM of Rapamycin for 4 hrs.

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