p53 Polyclonal Antibody/p53多克隆抗体

货号：49-1031

规格：50 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Raised against the C-term of human p53.

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	2.73 mg/mL	用法：	1-10 µg/4x10^6 cells(ChIP)；1:4000(ELISA)；1:2,000(WB)；1 µg/4x10^6 cells(ChIP-Seq)

靶标信息The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.

数据：

p53 Antibody (49-1031) in ChIPChIP assays were performed using the human osteosarcoma (U-2 OS) cells, the anti-p53 antibody (Product # 49-1031), and optimized PCR primer sets. Each ChIP assay used sheared chromatin from 1 million cells and 2 µg of anti-p53 antibody or beads only. This figure shows the recovery expressed as a percentage of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). Left: recovery by p53 of the p21 promoter, a known target for p53, or beads only. Recovery of the p21 promoter by the p53 antibody is evident based on fluorescent qPCR analysis of immunoprecipitated DNA. Right: recovery of the GAPDH promoter (used as a negative control) by p53 or beads only. p53 Antibody (49-1031) in ELISATo determine the titer of the antibody, an ELISA was performed using a serial dilution of anti-human p53 antibody (Product # 49-1031), crude serum and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:308,000.

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