BCL-2 Human ELISA Kit ELISA试剂盒,BCL-2 Human ELISA Kit/人BCL-2 ELISA试剂盒

2024-10-23

BCL-2 Human ELISA Kit/人BCL-2 ELISA试剂盒

货号:BMS244-3,BMS244-3TEN

规格:96 Tests,10X96 Tests

价格:1,35475

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

<0.5 ng/mL

检测范围0.50-32.0 ng/mL

样本类型/体积

血清 20 μL

血浆 20 μL

细胞裂解液 20 μL

操作时间

1 hr 20 min

测试时间

3 hr 10 min

Homogenous (no wash)

No

批间变异系数(CV)12%
批内变异系数(CV)8.6%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

10 x 96 tests

组份

- 预包被96孔板

- 标准品

- 样品稀释液

- 测定稀释浓缩液

- 生物素化检测抗体

-SAV-HRP

- 洗涤液

- 裂解液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameBCL-2
Protein aliasesApoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, protein phosphatase 1, regulatory subunit 50

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesBcl-2, BCL2, PPP1R50
Gene ID(Human) 596
Gene symbolBCL2
UniProt ID(Human) P10415
The Human Bcl-2 ELISA quantitates Hu Bcl-2 in human serum, plasma, or cell lysates. The assay will exclusively recognize both natural and recombinant Hu Bcl-2.人Bcl-2 ELISA可以定量人血清,血浆或细胞裂解物中的Bcl-2。 该检测试剂盒将专门识别天然和重组得人Bcl-2。Principle of the method 方法原理The Human Bcl-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人Bcl-2固相夹心ELISA(酶联免疫吸附测定)设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每批生产都经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.BCL-2是凋亡的关键调节因子,其功能是抑制或促进细胞死亡。BCL-2家族成员的特征还在于二聚化以进一步调节细胞凋亡。例如,已发现Bag1与BCL-2形成异二聚体,从而增强BCL-2的抗凋亡作用。已经表明,Bax和Bak在线粒体释放细胞色素c中起着关键作用,从而启动细胞凋亡。 Bax对细胞发挥促凋亡作用而不是抗凋亡作用。 BCL2的组成型表达,例如在BCL2易位至Ig重链基因座的情况下,被认为是滤泡性淋巴瘤的原因。在大多数滤泡性淋巴瘤中,肿瘤生发中心表达高水平的BCL-2α蛋白,而正常或增生生发中心为阴性。通过交替剪接产生的BCL-2的两个转录变体,其C末端不同。 BCL-2的过表达与人类癌症有关,例如B细胞淋巴瘤和前列腺癌。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

BCL-2 Human ELISA KitRepresentative standard curve for Human BCL-2 ELISA

技术参数
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