C3a Human ProcartaPlex™ Simplex Kit/人C3a ProcartaPlex单因子试剂盒

货号：EPX010-12282-901

规格：96 tests

价格：1

产品类型：多因子检测

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

Immunoassay Kit Grade:	Simplex Kit
测定范围:	As determined for Lot 1: 12.2-5000 pg/mL
Gene ID (Entrez):	718
样品体积:	CCS: 50 µL, Serum, Plasma: 25 µL
Interassay CV:	4.1%
Intraassay CV:	7.4%
样品类型:	Cell Culture Supernatants, Serum, Plasma
Assay Sensitivity:	0.6 pg/mL
Bead Region:	14
Gene Symbol:	C3
Former Identifiers:	eBioscience
Gene Alias:	AHUS5, ARMD9, ASPa, C3b, CPAMD1, HEL-S-62p, C3
基因:	complement C3
蛋白质:	Complement C3
Shipping Condition:	Wet Ice
Target Kit Named:	C3a
System Type:	Luminex™
Target Species Primary:	Human
Target List:	C3a
Target Species Named:	Human
Product Line:	ProcartaPlex™
UniProt ID:	P01024
Label or Dye:	R-PE
Immunoassay Kit Format:	Simplex Kit
Detection Method:	Fluorescent
适用于（设备）:	Luminex™ Instruments

The Human C3a Simplex ProcartaPlex Kit measures C3a protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.About ProcartaPlex Assays for the Luminex PlatformProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.•More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample• Well-established Luminex technology—highly referenced multiplexing platform for protein detection and quantitationProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 µL sample from plasma, serum, cell culture supernatants, and other bodily fluids.The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g. Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visitthermofisher.com/procartaplexfor more information, including a comprehensive list of individual protein targets.

技术参数

组成成分 • 1 vial Capture Beads (50X) • 1 vial biotinylated Detection Antibody (50X) • 2 vials Human Standard Mix C3a (lyophilized) • 1 bottle Universal Assay Buffer

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