蛋白质电泳marker,PageRuler™ Unstained High Range Protein Ladder

2024-10-23

PageRuler™ Unstained High Range Protein Ladder

货号:26637

规格:2 x 250 µL

价格:1079

产品类型:蛋白凝胶电泳

品牌:Thermo Fisher

Thermo Scientific PageRuler Unstained High Range Protein Ladder is a mixture of eight (8) unstained proteins (60 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.Features of PageRuler Unstained High Range Protein Ladder:• Size range—8 proteins spanning 60 to 250kDa• Ready-to-use—supplied in a loading buffer for direct loading on gels• Sharp bands—excellent accuracy for detection in with coomassie or silver stains• Reference band—150kDa band has greater intensity for easy orientation• Tagged—each protein in the ladder contains an integral Strep-tag™ II Sequence• Quality tested—each lot evaluated by SDS-PAGE and Western blottingThe protein MW markers in this ladder resolve into clearly identifiable sharp bands when analyzed by SDS-PAGE and stained with coomassie dye or silver. The 150kDa band is of greater intensity and serves as a reference band. Proteins can be detected on Western blots by staining with Ponceau S, coomassie blue or other protein stains. In addition, the ladder proteins contain an integral Step-tag™ II Sequence and may be detected on Western blots using Strep-Tactin™ Conjugates. The PageRuler Unstained High Range Protein Ladder is supplied ready-to-use: no heating, dilution or addition of a reducing agent is required before use.Includes:• Proteins (0.02 to 0.05 mg/mL of each) in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 100 mM DTT, 1 mM NaN3, 0.01% bromophenol blue and 33% glycerolApplications:• Accurate protein sizing on SDS-polyacrylamide gels and Western blots.
组分及储存条件:
Upon receipt store at -20°C. Product is shipped with an ice pack.

技术参数
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