人，CD34细胞培养，造血干细胞培养,StemMACS™ HSC Expansion Media, human-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

人，CD34细胞培养，造血干细胞培养,StemMACS™ HSC Expansion Media, human

2024-10-24

StemMACS™ HSC Expansion Media, human

货号：130-100-473,130-100-463

规格：100mL,500mL

价格：1342,0

产品类型：造血干细胞培养

品牌：Miltenyi

产品概述：StemMACS HSC Expansion Media XF (xeno-free) have been developed for the expansion of isolated CD34⁺ hematopoietic stem and progenitor cells. They are ideally suited for the expansion of CD34+ cells from cord blood, peripheral blood, or bone marrow.StemMACS HSC Expansion Media XF (xeno-free) is an optimized and standardized medium for the expansion of isolated CD34+ hematopoietic stem and progenitor cells. The media formulation is serum-free and xeno-free and is manufactured under strictly controlled conditions. StemMACS HSC Expansion Media XF offers consistent lot-to-lot performance. Recombinant growth factors, required for the optimal growth and expansion of hematopoietic stem cells (HSCs), have not been added to the media. They are available as separate product and have to be added prior to use. StemMACS HSC Expansion Cocktail (# 130-100-843) contains a combination of recombinant human cytokines designed to support the proliferation of human hematopoietic progenitor cells. It contains recombinant human stem cell factor (SCF), Flt3- ligand, and thrombopoietin (TPO). This combination of cytokines induces the proliferation of HSCs and immature progenitors.应用：Expansion of CD34+ cells from cord blood，peripheral blood，or bone marrowRetrovial transduction of CD34+ cells

优点：

-简单、快速；-无需分离柱；-分选细胞纯度可达98%；-与 SepTube配合使用可做快速、大量分选

数据：

图1.High CD34+ cell content:Cryopreserved CD34+cells separated from cord blood were expanded for 7 days in the indicated media supplemented with StemMACS HSC Expansion Cocktail (n=4; mean+/-SD). StemMACS HSC Expansion Media XF performed consistently best in maintaining high levels of CD34+ cells during expansion.

图2.Broad differentiation potential:

Isolated CD34+ cord blood cells were expanded in the indicated media plus StemMACS HSC Expansion Cocktail. After 7 days, cells were analyzed for their CFU potential. Cells cultured in StemMACS HSC Expansion Media XF maintained broad hematopoietic differentiation potential.

技术参数

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人，CD34细胞培养，造血干细胞培养,StemMACS™ HSC Expansion Media, human

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