Pierce™ Detergent Compatible Bradford Assay Kit,Pierce™ Detergent Compatible Bradford Assay Kit/兼容洗涤剂的Bradford检测试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Pierce™ Detergent Compatible Bradford Assay Kit,Pierce™ Detergent Compatible Bradford Assay Kit/兼容洗涤剂的Bradford检测试剂盒

2024-10-23

Pierce™ Detergent Compatible Bradford Assay Kit/兼容洗涤剂的Bradford检测试剂盒

货号：23246

规格：300 test tube assays

价格：2153

产品类型：蛋白定量

品牌：Thermo Fisher

The Pierce Detergent Compatible Bradford Assay Kit is a quick and ready-to-use modification of the well-known Bradford Coomassie dye-binding, colorimetric method for total protein quantitation. Unique additives to the Bradford Reagent make it compatible with up to 1% or higher of detergents and lysis reagents that are commonly used in life science research, including Triton X-100 and NP-40.•Flexible—compatible with samples both with and without detergent•Sample volume—requires only 10 µL for microplate procedure•Colorimetric—measure with a standard spectrophotometer or plate reader at 595 nm•Ready-to-use—single reagent; no working reagent preparation required•Assay time—10-min incubation at room temperature•Assay range—detects protein concentration in the range of 2 to 1500 µg/mLSimilar to the Bradford method, Coomassie dye binds protein in an acidic medium causing an immediate shift in absorption maximum from 465 nm to 595 nm with a concomitant color change from green to blue. In addition, the assay is complete in just 10 minutes.The protein assay can be performed in either test tube or microplate format. The standard working range is 100–1500 µg/mL with up to 1% detergent (or higher in some cases). Protein concentrations are estimated by reference to absorbances obtained for a series of standard protein dilutions, typically bovine serum albumin (BSA), which are assayed alongside the unknown samples. Because the color response with Coomassie dye is non-linear with increasing protein concentration, a standard curve must be completed with each assay. Standards can be used directly without preparing them in the same detergent found in the test samples.

数据：

The Pierce Detergent Compatible Bradford Assay provides advantages over the DC Protein Assay

Superior detergent compatibility with the Pierce Detergent Compatible Bradford Protein AssayAssays were performed according to the microplate procedure using BSA standards spiked with detergent or water (control). Sample and reagent volumes used were according to the manufacturer's instructions. Each assay was incubated for 10 minutes and absorbance was measured at 595nm. The Pierce Detergent Compatible Bradford Assay was found to be compatible with 11 commonly used detergents. Compatibility is defined as < 10% difference in absorbance between samples with and without detergent. In contrast, the traditional Bio-Rad Quick Startª Bradford Protein Assay was found to be compatible with only three of the 11 detergents tested.

Better sensitivity with the Pierce Detergent Compatible Bradford Protein Assay versus the Bio-Rad DC Protein AssayEach assay was performed in a microplate using BSA standards spiked with detergent or water (control), and followed the manufacturer's instructions. More importantly, the range of the standard curve for the Pierce Assay was four times broader than the range for the DC™ Assay.

技术参数

组成成分 Detergent Compatible Bradford Assay Reagent, 450 mL, store at 4°C Albumin Standard Ampules, 10 x 1 mL, store at room temperature

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Pierce™ Detergent Compatible Bradford Assay Kit,Pierce™ Detergent Compatible Bradford Assay Kit/兼容洗涤剂的Bradford检测试剂盒

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