Qubit™ Protein Assay Kit,Qubit™ Protein Assay Kit/Qubit蛋白测定试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Qubit™ Protein Assay Kit,Qubit™ Protein Assay Kit/Qubit蛋白测定试剂盒

2024-10-23

Qubit™ Protein Assay Kit/Qubit蛋白测定试剂盒

货号：Q33211,Q33212

规格：100 assays,500 assays

价格：1468,4436

产品类型：蛋白定量

品牌：Thermo Fisher

The Qubit Protein Assay Kit is designed specifically for use with the Qubit Fluorometer. Using between 1 and 20 µl of your sample, this assay can quantitate samples ranging from 12.5 µg⁄ml to 5 mg⁄ml and exhibits low protein to protein variation. The assay is highly selective for proteins and is designed to be accurate in the presence of reducing reagents, but not in the presence of a large amount of detergent. Common contaminants, such as reducing reagents (DTT, β-mercaptoethanol), salts, free nucleotides, amino acids, solvents, or DNA, but not detergents, are well tolerated in the assay. Slight protocol modifications are required for other contaminants. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted BSA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration.Which product to choose for fluorometric protein quantitation?• For 1–20 samples: use this Qubit Protein Assay Kit with the Qubit Fluorometer• For 20–2000 samples: use the Quant-iT Protein Assay Kit with microplate readerNotes:1. All Qubit assay kits can be used with the Qubit 1.0, Qubit 2.0, Qubit 3, and Qubit 4 fluorometers.2.500 µL thin-walled PCR tubesare required but not included.

数据：

Qubit Protein Assays display higher accuracy, precision, and sensitivity than 3 other common protein assays.The Qubit® Protein Assay with the Qubit® 2.0 Fluorometer produces less protein-to-protein variation and higher accuracy, precision, and sensitivity than 3 other common protein assays (A–D). The same lot of each protein was used in all assays, and assays were carried out in triplicate following the manufacturers’ protocols. Data are graphed to show protein-to-protein variation throughout the protein concentration range tested. The inset in (A) is a magnification of the low end of the protein range, to show the sensitivity of the Qubit® Protein Assay used with the Qubit® 2.0 Fluorometer.

Low protein-to-protein variation in the Qubit® protein assayLow protein-to-protein variation in the Qubit® protein assay (Q33211, Q33212). Solutions of the following proteins were prepared, diluted, and assayed in the Qubit® protein assay: bovine serum albumin (BSA), chicken-egg ovalbumin, chicken-egg lysozyme, bovine-milk ß-casein, equine myoglobin, bovine-milk a-casein, porcine pepsin, mouse immunoglobulin (IgG), and calf-thymus histone. Fluorescence was measured at 485/590 nm and plotted versus the mass of the protein sample. At 3 µg, the fluorescence variation was 12.4%, or 8.7% excluding the basic histone protein. Background fluorescence has not been subtracted.

技术参数

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Qubit™ Protein Assay Kit,Qubit™ Protein Assay Kit/Qubit蛋白测定试剂盒

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