Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, HRP

货号：A16110,A16110SAMPLE

规格：1 mg,50 µg

价格：1983,436

产品类型：二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobins Heavy and Light chains

物种：兔

宿主：山羊

抗体亚型：其它

荧光染料：其它

类型:	二抗	同型对照：
浓度：		用法：	1:500-1:20,000(ELISA)；1:500-1:20,000(IHC)；1:10,000-1:200,000(WB)

靶标信息Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据：

Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A16110) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1) and Jurkat (Lane 2). The blots were probed with Anti-CacyBP Rabbit Polyclonal Antibody (Product # 720326, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, HRP (Product # A16110) at dilutions 1:10,000 (Fig. 1), 1:100,000 (Fig. 2) and 1:200,000 (Fig. 3). A 30 kDa band corresponding to CacyBp was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

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参考文献：

1. Frontiers in surgeryThe Identification of Three Cancer Stem Cell Subpopulations within Moderately Differentiated Lip Squamous Cell Carcinoma.2. Frontiers in surgeryGlioblastoma Multiforme Cancer Stem Cells Express Components of the Renin-Angiotensin System.3. Frontiers in surgeryCancer Stem Cells in Moderately Differentiated Buccal Mucosal Squamous Cell Carcinoma Express Components of the Renin-Angiotensin System.4. Molecular medicine reportsEffects of Hylomecon vernalis ethanol extracts on cell cycle and apoptosis of colon cancer cells.5. Oncology lettersDeregulation of miR-193b affects the growth of colon cancer cells via transforming growth factor-β and regulation of the SMAD3 pathway.

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