Pierce™ Protein Concentrator PES, 30K MWCO, 20-100 mL,Pierce™ Protein Concentrator PES, 30K MWCO, 20-100 mL/蛋白浓缩管-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Pierce™ Protein Concentrator PES, 30K MWCO, 20-100 mL,Pierce™ Protein Concentrator PES, 30K MWCO, 20-100 mL/蛋白浓缩管

2024-10-23

Pierce™ Protein Concentrator PES, 30K MWCO, 20-100 mL/蛋白浓缩管

货号：88536

规格：4 reactions

价格：2461

产品类型：蛋白提取/纯化

品牌：Thermo Fisher

Thermo Scientific™ Pierce™ Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples. This product has a molecular weight cutoff (MWCO) of 30K and can be used for processing sample volumes ranging from 20 to 100 mL.Features of Pierce Protein Concentrators PES:• Rapid protein concentration—sample concentration of 10- to 30-fold can be achieved in 5 to 30 minutes for 10K MWCO (time may vary for a product with a different MWCO)• Excellent protein recovery—typical protein recovery is >90%• Versatility—perform sample concentration, diafiltration, or buffer-exchange of biological samples• Compatibility—may be used in both fixed angle and swing bucker rotors; collect and recover sample without invert spinningFor easy identification, the MWCO value is etched on the side of each device. To assist with sample addition and recovery, a clear window with graduations is located on the side of each device enabling visual determination of the retentate volume. The vertical design of the membrane allows high flux rates, low nonspecific binding, and negligible protein aggregation on the membrane; and it offers reliable and consistent results.Pierce Protein Concentrators PES contain a membrane rated for retaining molecules with a molecular weight at least 2-fold greater than the MWCO rating of the PES membrane within the device. Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass. Protein recovery will vary depending on the specific protein in the sample and its starting concentration. To achieve >90% recovery of protein, the minimum protein sample concentration should be 0.1 mg/mL.

数据：

Comparison of flux curves for different MWCOs using 20-100 mL concentratorsProtein samples for each MWCO (5K, 10K, 30K, 100K) at approximately 0.25 mg/mL starting concentration were centrifuged in 20-100 mL Pierce Protein Concentrators PES at 1200 x g for 90 min to determine the protein concentration rate. For 10K, 30K, and 100K MWCOs, >90% of the protein solution was concentrated within 15 min; for the 5K MWCO, this degree of concentration occurred within 90 min.

Processing time and protein recovery using 20-100 mL protein concentrators90 mL volumes of four different protein solutions were concentrated over 30-fold in a swinging bucket rotor at 1200 x g at 25°C, using 5K, 10K, 30K, or 100K MWCO Pierce Protein Concentrators PES (20-100 mL).

技术参数

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Pierce™ Protein Concentrator PES, 30K MWCO, 20-100 mL,Pierce™ Protein Concentrator PES, 30K MWCO, 20-100 mL/蛋白浓缩管

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