E-selectin (Soluble) Human ELISA Kit ELISA试剂盒,E-selectin (Soluble) Human ELISA Kit/人E-选择素（可溶）ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

E-selectin (Soluble) Human ELISA Kit ELISA试剂盒,E-selectin (Soluble) Human ELISA Kit/人E-选择素（可溶）ELISA试剂盒

2024-10-23

E-selectin (Soluble) Human ELISA Kit/人E-选择素（可溶）ELISA试剂盒

货号：BMS205,BMS205TEN

规格：96 Tests,10X96 Tests

价格：1,34832

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	0.3 ng/mL
检测范围	1.6-50 ng/mL
样本类型/体积	血清 20 μL 血浆 20 μL 上清液 20 μL
操作时间	1 hr 20 min
测试时间	2 hr 10 min
Homogenous (no wash)	No
批间变异系数（CV）	6%
批内变异系数（CV）	5.4%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - 生物素化检测抗体 - SAV-HRP - 洗涤液 - 对照 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	E-selectin (CD62E)
Protein aliases	CD62 antigen-like family member E, CD62E, E-selectin, ELAM-1, endothelial adhesion molecule 1, Endothelial leukocyte adhesion molecule 1, LECAM2, leukocyte endothelial cell adhesion molecule 2, Leukocyte-endothelial cell adhesion molecule 2
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	HRP
Gene aliases	CD62E, ELAM, ELAM1, ESEL, LECAM2, SELE
Gene ID	(Human) 6401
Gene symbol	SELE
UniProt ID	(Human) P16581

The Human e-Selectin/Endothelial Leukocyte Adhesion Molecule-1 (Hu ELAM-1) ELISA quantitates Hu ELAM-1 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu ELAM-1.人e-选择素/内皮白细胞粘附分子1（人ELAM-1）ELISA定量了人血清，血浆，缓冲液或细胞培养基中的人ELAM-1。该检测试剂盒将专门识别天然和重组人Elam-1。Principle of the method 方法原理The Human ELAM-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人ELAM-1固相夹心ELISA（酶联免疫吸附测定）旨在测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每批生产都经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.白细胞在毛细血管后小静脉的淋巴结外渗期间粘附在血管内皮上。另外，炎症发作后，白细胞粘附发生在身体任何部位的毛细血管和小静脉中。这种反应是即时的，涉及一系列的粘附受体。在选择蛋白和免疫球蛋白超家族的内皮表面成员参与该级联反应。选择素是C型细胞表面凝集素，在白细胞粘附于血管壁内皮细胞中发挥作用。 E-选择素（CD62E）是一种内皮细胞特异性选择素，仅在被促炎性细胞因子激活后才表达。体外实验表明，IL-1，TNFα和细菌壁成分（如脂多糖）在依赖NFkB的信号级联反应中诱导E-选择蛋白的转录。 E-选择蛋白已在多种炎症情况下与血管内皮相关。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

E-selectin (Soluble) Human ELISA KitRepresentative standard curve for Human soluble e-Selectin ELISA

技术参数

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E-selectin (Soluble) Human ELISA Kit ELISA试剂盒,E-selectin (Soluble) Human ELISA Kit/人E-选择素（可溶）ELISA试剂盒

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