APO-1/FAS (Soluble) Human ELISA Kit ELISA试剂盒,APO-1/FAS (Soluble) Human ELISA Kit/人APO-1/FAS (可溶)ELISA试剂盒

2024-10-23

APO-1/FAS (Soluble) Human ELISA Kit/人APO-1/FAS (可溶)ELISA试剂盒

货号:BMS245,BMS245TEN

规格:96 Tests,10X96 Tests

价格:1,35475

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

13.2 pg/mL

检测范围15.6-1,000 pg/mL

样本类型/体积

血清 10 μL

上清液 10 μL

操作时间

1 hr 20 min

测试时间

2 hr 10 min

Homogenous (no wash)

No

批间变异系数(CV)3.1%
批内变异系数(CV)4.5%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

10 x 96 tests

组份

- 预包被96孔板

- 标准品

- 样品稀释液

- 测定稀释浓缩液

- 生物素化检测抗体

- SAV-HRP

- 洗涤液

- 对照

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameFAS (CD95)
Protein aliasesApo-1 antigen, Apoptosis-mediating surface antigen FAS, FASLG receptor, APO-1 cell surface antigen, apoptosis antigen 1, apoptosis signaling receptor FAS, CD95, CD95 antigen, Fas (TNF receptor superfamily, member 6), Fas AMA, TNF receptor superfamily member 6, Tumor necrosis factor receptor superfamily member 6, tumor necrosis factor receptor superfamily, member 6

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesALPS1A, APO-1, APT1, CD95, FAS, FAS1, FASTM, TNFRSF6
Gene ID(Human) 355
UniProt ID(Human) P25445
Gene symbolFAS

The Human Soluble APO-1/Fas ELISA quantitates Hu sAPO-1/Fas in human serum, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu sAPO-1/Fas.人可溶性APO-1 / Fas ELISA定量了人血清或细胞培养基中的人sAPO-1 / Fas。 该检测试剂盒将专门识别天然和重组r 人sAPO-1 / Fas。Principle of the method 方法原理The Human APO-1/Fas solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人APO-1 / Fas固相夹心ELISA(酶联免疫吸附测定)旨在测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每批生产都经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. FAS also exists in a soluble form that weight approximately 26kDa. Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases. The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis. FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform. The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer. It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.FAS(Fas,CD95,APO-1)是一种46 kDa的跨膜糖蛋白,起TNFR(肿瘤坏死因子受体)超家族的细胞死亡受体的作用。 FAS也以重量约26kDa的可溶形式存在。 FAS的刺激导致其细胞内FAS相关死亡域(FADD)的聚集,诱导死亡的信号复合物(DISC)的形成和胱天蛋白酶的激活。复合物中半胱氨酸蛋白酶的自动蛋白水解过程触发下游级联反应,导致细胞凋亡。已证明FAS激活NF-κB,MAPK3 / ERK1,MAPK8 / JNK,并参与正常二倍体成纤维细胞和T细胞中增殖信号的转导。已经描述了编码七个不同同工型的至少八个选择性剪接的转录变体。缺少跨膜结构域的同工型可能负调控全长同工型介导的细胞凋亡。 Fas / Fas配体系统已显示出在许多人类疾病(如艾滋病,肝炎和癌症)中的作用。还假定通过FAS配体途径诱导的细胞凋亡主要参与抗病毒免疫应答。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

APO-1/FAS (Soluble) Human ELISA KitRepresentative standard curve for Human APO-1/FAS ELISA

技术参数
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