FABP-3 Human ELISA Kit/人FABP-3 ELISA试剂盒
货号：BMS2263,BMS2263TEN
规格：96 Tests,10X96 Tests
价格：7202,35475
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 26.88 pg/mL |
| 检测范围 | 156.3-5000 pg/mL |
样本类型/体积 | 血清 50 μL 血浆 50 μL 上清液 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr |
Homogenous (no wash) | No |
| 批间变异系数（CV） | 6.2% |
| 批内变异系数（CV） | 3.9% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 10 x 96 tests |
组份 | - 预包被96孔板 - 标准品 - 标准稀释液 - 测定稀释浓缩液 - 生物素化检测抗体 - SAV-HRP - HRP稀释液 - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | FABP3 |
| Protein aliases | fatty acid binding protein 11, fatty acid binding protein 3, muscle and heart, Fatty acid-binding protein 3, Fatty acid-binding protein, heart, Heart-type fatty acid-binding protein, H-FABP, Mammary-derived growth inhibitor, MDGI, Muscle fatty acid-binding protein, M-FABP |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | FABP11, FABP3, H-FABP, M-FABP, MDGI, O-FABP |
| Gene ID | (Human) 2170 |
| UniProt ID | (Human) P05413 |
| Gene symbol | FABP3 |
The Human FABP-3 ELISA quantitates Hu FABP-3 in human serum, plasma or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu FABP-3.人FABP-3 ELISA定量了人血清，血浆或细胞培养基中的人FABP-3。 该检测试剂盒将专门识别天然和重组人FABP-3。Principle of the method 方法原理The Human FABP-3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人FABP-3固相夹心ELISA（酶联免疫吸附测定）旨在测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二个（检测）抗体形成三明治，该第二个抗体与捕获抗体在不同表位上的靶标结合。 已将结合酶掺入测定中。 经过孵育和洗涤步骤以去除未结合物质的微孔板后，添加底物溶液，该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每批生产都经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Fatty acid binding proteins (FABP) are small (approximately 13-14 kDa) intracellular proteins with a high degree of tissue specificity. FABPs are a class of cytoplasmic proteins that bind long chain fatty acids. They are abundantly present in various cell types and seem to play an important role in the intracellular utilization of fatty acids. There are at least six distinct types of FABP, each showing a specific pattern of tissue expression. FABP leaks, due to its small size, rapidly out of is chemically damaged dying cells leading to a rise in serum levels. Liver FABP is a sensitive marker for cell damage of liver cells in vitro and in vivo. Is chemically damaged tissues are characterized histologically by absence (or low presence) of FABP facilitating recognition of such areas. Next to this L FABP is a marker for rapid hepatocyte lysis in vitro (as for example in toxicology assays) and for detection of liver damage during and after transplantation.脂肪酸结合蛋白（FABP）是小的（约13-14 kDa）细胞内蛋白，具有高度的组织特异性。 FABP是结合长链脂肪酸的一类细胞质蛋白。 它们大量存在于各种细胞类型中，似乎在脂肪酸的细胞内利用中起重要作用。 至少有六种不同类型的FABP，每种都显示出特定的组织表达模式。 FABP泄漏由于其体积小而迅速从化学损伤的垂死细胞中消失，导致血清水平升高。 肝FABP是体外和体内肝细胞损伤的敏感标志物。 化学损伤的组织在组织学上的特征是不存在FABP（或存在率低），从而有助于识别这些区域。 L FABP的旁边是体外快速肝细胞裂解（例如在毒理学测定中）以及在移植期间和移植后检测肝损伤的标记。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
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