| The Mouse Interleukin-17F (Ms IL-17F) ELISA quantitates Ms IL-17F in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IL-17F.小鼠白介素-17F（小鼠 IL-17F）ELISA定量检测小鼠血清，血浆，缓冲液或细胞培养基中的小鼠IL-17F。 该检测试剂盒将专门识别天然和重组小鼠IL-17F。Principle of the method 方法原理The Mouse IL-17F solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.小鼠IL-17F固相夹心ELISA（酶联免疫吸附测定）旨在测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每批生产都经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息IL-17F (Interleukin 17F, CTLA-8)) is a cytokine belonging to the IL-17 family that is produced by inflammatory cells such as activated T cells, mast cells, and basophils. IL-17F is involved in allergic airway inflammation, and can induce several cytokines, chemokines, and adhesion molecules in bronchial epithelial cells, vein endothelial cells, fibroblasts, and eosinophils. IL-17F may be secreted as a homodimer, or a heterodimer with IL17A. It acts by binding to the type I receptor, IL-17R, aiding recruitment of monocytes and neutrophils at the site of inflammation by increasing chemokine production. IL-17F also stimulates induction of other pro-inflammatory cytokines TNF alpha, IL-1 beta, IL-6, and IL-8, and reports strongly suggest the involvement of IL-17 in several chronic inflammatory diseases such as rheumatoid arthritis, psoriasis and multiple sclerosis. TGF-beta (differentiation) and IL-23 (expansion) are required for induction and maintenance of Th17 (IL-17 producing) cells, which in turn induce the other pro-inflammatory cytokines. IL-17F is produced, and exists, as a homo-dimer, with homology to a herpes virus early protein, is one of the six members (IL-17A-F) of this cytokine family, and is well characterized and highly expressed by activated effector memory T cells. IL-17F has been found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1.IL-17F（白介素17F，CTLA-8）是属于IL-17家族的一种细胞因子，是由诸如活化T细胞，肥大细胞和嗜碱性粒细胞之类的炎性细胞产生的。 IL-17F与过敏性气道炎症有关，可在支气管上皮细胞，静脉内皮细胞，成纤维细胞和嗜酸性粒细胞中诱导多种细胞因子，趋化因子和粘附分子。 IL-17F可能被分泌为IL17A的同二聚体或异二聚体。它通过与I型受体IL-17R结合而起作用，通过增加趋化因子的产生来帮助在炎症部位募集单核细胞和中性粒细胞。 IL-17F还刺激其他促炎性细胞因子TNFα，IL-1 beta，IL-6和IL-8的诱导，并且有报道强烈暗示IL-17参与了几种慢性炎症性疾病，如类风湿性关节炎，牛皮癣和多发性硬化症。 TGF-beta（分化）和IL-23（扩增）是诱导和维持Th17（产生IL-17的细胞）所必需的，而Th17细胞又可以诱导其他促炎性细胞因子。 IL-17F产生并以与疱疹病毒早期蛋白同源的同型二聚体形式存在，是该细胞因子家族的六种成员之一（IL-17A-F），并具有良好的特征和表达能力激活的效应记忆T细胞。已经发现IL-17F抑制内皮细胞的血管生成并诱导内皮细胞产生IL2，TGFB1 / TGFB和单核细胞趋化蛋白-1。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。 |
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