Myoglobin Human ELISA Kit/人肌红蛋白ELISA试剂盒
货号：BMS2259,BMS2259TEN
规格：96 Tests,10X96 Tests
价格：1,1
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 0.039 ng/mL |
| 检测范围 | 0.39–25 ng/mL |
样本类型/体积 | 血清 10 μL 血浆 10 μL 细胞裂解液 10 μL |
操作时间 | 1 hr 20 min |
测试时间 | 3 hr 30 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | 3.9% |
| 批内变异系数（CV） | 5.7% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 10 x 96 tests |
组份 | - 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - 生物素化检测抗体 - SAV-HRP - 洗涤液 - 底物溶液 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | Myoglobin |
| Protein aliases | Mb, MB, PVALB, myoglobgin, Myoglobin |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | MB, myoglobgin, PVALB |
| Gene ID | (Human) 4151 |
| Gene symbol | MB |
| UniProt ID | (Human) P02144 |
The Myoglobin Human ELISA Kit quantitates Hu Myoglobin in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Myoglobin.人肌红蛋白ELISA试剂盒可定量人血清，血浆或细胞培养基中的人肌红蛋白。 该检测试剂盒将只识别天然和重组的人肌红蛋白。Principle of the method 方法原理The Human Myoglobin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人肌红蛋白固相夹心ELISA（酶联免疫吸附测定）旨在测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二个（检测）抗体形成三明治，该第二个抗体与捕获抗体在不同表位上的靶标结合。 已将结合酶掺入测定中。 经过孵育和洗涤步骤以去除未结合物质的微孔板后，添加底物溶液，该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每批生产都经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a hemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported.该基因编码球蛋白超家族的一个成员，并在骨骼肌和心肌中表达。 编码的蛋白是一种血蛋白，有助于细胞内氧气的储存和氧气的跨细胞扩散。 已经报道了至少三种编码相同蛋白质的选择性剪接的转录变体。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
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