MCP-1 Human ELISA Kit ELISA试剂盒,MCP-1 Human ELISA Kit/人MCP-1 ELISA试剂盒

2024-10-23

MCP-1 Human ELISA Kit/人MCP-1 ELISA试剂盒

货号:BMS281,BMS281TEN

规格:96 Tests,10X96 Tests

价格:1,34832

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

2.3 pg/mL

检测范围15.6-1,000 pg/mL

样本类型/体积

血清 20 μL

血浆 20 μL

羊水 20 μL

上清液 20 μL

操作时间

1 hr 20 min

测试时间

2 hr 10 min

Homogenous (no wash)

No

批间变异系数(CV)8.7%
批内变异系数(CV)4.7%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

10 x 96 tests

组份

- 预包被96孔板

- 标准品

- 样品稀释液

- 测定稀释浓缩液

- HRP偶联检测抗体

- 洗涤液

- 对照

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameCCL2 (MCP-1)
Protein aliasesC-C motif chemokine 2, chemokine (C-C motif) ligand 2, HC11, MCAF, MCP-1, Monocyte chemoattractant protein 1, monocyte chemoattractant protein-1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je), small inducible cytokine subfamily A (Cys-Cys), member 2, Small-inducible cytokine A2

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物HRP
Gene aliasesCCL2, GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, SCYA2, SMC-CF
Gene ID(Human) 6347
Gene symbolCCL2
UniProt ID(Human) P13500

The Human Monocyte Chemoattractant Protein-1(Hu MCP-1) ELISA quantitates Hu MCP-1 in human serum, plasma,amniotic fluid, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MCP-1.人单核细胞趋化蛋白1(人MCP-1)ELISA定量测定人血清,血浆,羊水或细胞培养基中的人MCP-1。 该检测试剂盒将专门识别天然和重组人MCP-1。Principle of the method 方法原理The Human MCP-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人MCP-1固相夹心ELISA(酶联免疫吸附测定)旨在测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每批生产都经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.该基因是聚集在17号染色体q臂上的几种细胞因子基因之一。细胞因子是分泌蛋白家族,参与免疫调节和炎症过程。 该基因编码的蛋白质在结构上与细胞因子的CXC亚家族有关。 该亚家族的成员的特征是两个半胱氨酸被一个氨基酸隔开。 该细胞因子显示出对单核细胞和嗜碱性粒细胞的趋化活性,但对嗜中性粒细胞或嗜酸性粒细胞没有趋化活性。 它与牛皮癣,类风湿性关节炎和动脉粥样硬化等以单核细胞浸润为特征的疾病的发病机理有关。 它与趋化因子受体CCR2和CCR4结合。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权,不得转售。
数据:

MCP-1 Human ELISA KitRepresentative standard curve for Human MCP-1 ELISA

技术参数
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