p53 Human ELISA Kit ELISA试剂盒,p53 Human ELISA Kit/人p53 ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

p53 Human ELISA Kit ELISA试剂盒,p53 Human ELISA Kit/人p53 ELISA试剂盒

2024-10-23

p53 Human ELISA Kit/人p53 ELISA试剂盒

货号：BMS256,BMS256TEN

规格：96 Tests,10X96 Tests

价格：1,1

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	0.33 U/mL
检测范围	0.78-50 U/mL
样本类型/体积	血清 50 μL 上清液 50 μL
操作时间	1 hr 20 min
测试时间	3 hr 10 min
Homogenous (no wash)	No
批间变异系数（CV）	8.9%
批内变异系数（CV）	5.5%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - 生物素化检测抗体 - SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	p53
Protein aliases	Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, Cellular tumor antigen p53, mutant tumor protein 53, p53 tumor suppressor, transformation-related protein 53, tumor protein 53, tumor supressor p53
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	BCC7, LFS1, P53, TP53, TRP53
Gene ID	(Human) 7157
Gene symbol	TP53
UniProt ID	(Human) P04637

The Human p53 ELISA quantitates Hu p53 in human serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu p53.人p53 ELISA可以定量人血清或细胞培养基中的人p53。该检测试剂盒将只识别天然和重组人p53。Principle of the method 方法原理The Human p53 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人p53固相夹心ELISA（酶联免疫吸附测定）旨在测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每批生产都经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.肿瘤抑制蛋白p53是一种序列特异性转录因子，可被细胞应激激活。 p53对嘧啶核苷酸的DNA损伤或饥饿反应介导细胞周期停滞或凋亡。 p53响应应激信号而被上调，并被刺激激活特定基因的转录，从而导致p21waf1和其他参与G1或G2 / M阻滞的蛋白质的表达。 p53的结构包括N端反式激活结构域，中央DNA结合结构域，寡聚结构域和C端调节结构域。 p53上有多个磷酸化位点，其中Ser15处的磷酸化对于p53激活和稳定很重要。 p53的特征是在阻断受损细胞的增殖作用中发挥作用，并作为抗癌剂。已经显示p53中Ser392的磷酸化与人类肿瘤的形成有关。此外，p53还与衰老和氧化应激的影响有关，p53的增加与学习和记忆中LTP（长期增强）的缺陷有关。在正常细胞中发现p53的水平非常低，但是，在多种转化的细胞系中，p53的表达量很高，据信有助于转化和恶性肿瘤。在许多不同的人类癌症中频繁出现的p53突变体无法结合共有DNA结合位点，并导致肿瘤抑制活性丧失。 TP53基因的改变不仅在人类恶性肿瘤中以体细胞突变的形式出现，而且在一些易患癌症的家族中发生了种系突变，例如Li-Fraumeni综合征。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

p53 Human ELISA KitRepresentative standard curve for Human p53 ELISA

技术参数

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p53 Human ELISA Kit ELISA试剂盒,p53 Human ELISA Kit/人p53 ELISA试剂盒

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