Perforin Human ELISA Kit/人穿孔素ELISA试剂盒
货号：BMS2306,BMS2306TEN
规格：96 Tests,10X96 Tests
价格：5997,35475
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 11.8 pg/mL |
| 检测范围 | 28.1–1800 pg/mL |
样本类型/体积 | 血清 20 μL 血浆 20 μL 细胞裂解液 20 μL |
操作时间 | 1 hr 20 min |
测试时间 | 3 hr 30 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | 9.3% |
| 批内变异系数（CV） | 4.9% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 10 x 96 tests |
组份 | - 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - 生物素化检测抗体 - SAV-HRP - 洗涤液 - 底物溶液 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
| Protein name | Perforin |
| Protein aliases | Cytolysin, cytolysin, lymphocyte pore-forming protein, perforin 1 (pore forming protein), P1, PFP, HPLH2, lymphocyte pore forming protein, Lymphocyte pore-forming protein, Perforin-1 |
物种（Tested） | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | FLH2, HPLH2, P1, PFN1, PFP, PRF1 |
| Gene ID | (Human) 5551 |
| Gene symbol | PRF1 |
| UniProt ID | (Human) P14222 |
The Perforin Human ELISA Kit quantitates Hu Perforin in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Perforin.人穿孔素ELISA试剂盒可定量测定人血清，血浆或细胞培养基中的人穿孔素。该检测试剂盒将专门识别天然和重组的人穿孔素。Principle of the method 方法原理The Human Perforin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人穿孔素固相夹心ELISA（酶联免疫吸附测定）旨在测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二个（检测）抗体形成三明治，该第二个抗体与捕获抗体在不同表位上的靶标结合。 已将结合酶掺入测定中。 经过孵育和洗涤步骤以去除微孔板中未结合的物质后，添加底物溶液，该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每批生产都经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Perforin is one of the major cytolytic proteins of cytolytic granules. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Moreover, perforin is involved in immune defense against tumors and virus infections as mediated by cytotoxic lymphocytes. Perforin is a 555 amino acid protein with a 21 amino acid signal peptide, and has a molecular weight of 70 to 75 kD. Perforin is a pore forming protein with a mechanism of transmembrane channel formation similar to C9, and homology between perforin and C9 have been demonstrated. Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested. Perforin is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in the perforin gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants of perforin.穿孔素是溶细胞颗粒的主要溶细胞蛋白之一。穿孔素是一种细胞溶解介质，被储存在细胞质颗粒中并由其释放。而且，穿孔素参与了由细胞毒性淋巴细胞介导的针对肿瘤和病毒感染的免疫防御。穿孔素是具有21个氨基酸的信号肽的555个氨基酸的蛋白，分子量为70至75 kD。穿孔素是具有类似于C9的跨膜通道形成机制的成孔蛋白，并且已经证明穿孔素和C9之间的同源性。研究表明，穿孔素仅在杀伤细胞系中表达，而在辅助T淋巴细胞或其他测试的肿瘤细胞中不表达。穿孔素已知是T细胞和自然杀伤细胞介导的细胞溶解的关键效应分子。穿孔素基因的缺陷会导致2型家族性噬血细胞淋巴组织细胞增生症（HPLH2），这是一种罕见的致命性常染色体早幼性隐性疾病。选择性剪接导致穿孔蛋白的多个转录变体。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权，不得转售。
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