IL-4 Bovine Uncoated ELISA Kit ELISA试剂盒,IL-4 Bovine Uncoated ELISA Kit/牛IL-4未包被的ELISA试剂盒

2024-10-23

IL-4 Bovine Uncoated ELISA Kit/牛IL-4未包被的ELISA试剂盒

货号:ESS0031

规格:5 x 96 tests

价格:7267

产品类型:自定义ELISA

品牌:Thermo Fisher

物种:其它

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

<15.6 pg/mL

检测范围15.6-1000 pg/mL

样本类型/体积

血清 100 μL

上清液 100 μL

操作时间

24 hr

测试时间

3 hr 20 min

Homogenous (no wash)

No

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

5 x 96 tests

组份

- 包被抗体

- 冻干重组标准

- 检测抗体

- 链霉亲和素-HRP

- 底物溶液

- 终止溶液,br> 0.16M硫酸

- 分析证书:特定批次的抗体和标准品稀释说明

储存温度

2-8℃

Protein nameIL-4
Protein aliasesB-cell stimulatory factor 1, BSF-1, IL-4, Interleukin-4, Lymphocyte stimulatory factor 1

物种(Tested)

试剂盒类型

Uncoated ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesBOS_7769, BSF-1, IL-4, IL4
Gene ID(Bovine) 280824
Gene symbolIL4
UniProt ID(Bovine) P30367

The Bovine IL-4 Uncoated ELISA Kit contains pre-matched antibody pairs and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of bovine IL-4.牛IL-4未包被ELISA试剂盒包含预先匹配的抗体对和试剂,用于进行定量酶联免疫吸附测定(ELISA),以检测和定量牛IL-4的蛋白质水平。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA用于测量匹配的抗体对之间结合的靶标的量。 将靶标特异性抗体包被在微孔板孔的底部,这是一整夜的过程。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.白介素-4(IL-4)是由活化的T细胞产生的多效性免疫调节细胞因子,可作为白介素4受体的配体。 已经发现IL-4具有最广泛的作用范围。 白介素4受体也与IL13结合,这可能导致该细胞因子和IL13的许多重叠功能。 STAT6是一种信号转导和转录激活因子,已显示在介导IL-4的免疫调节信号中起重要作用。 IL-4基因,IL-3,IL-5,IL-13和CSF2在5q染色体上形成细胞因子基因簇。 特别地,IL-4在染色体上紧邻IL13。 此外,发现IL-4基因,IL13和IL5由染色体上120kb范围内的几个远程调控元件协调。 IL-4δ2是IL-4的已知剪接变体。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
数据:

IL-4 Bovine Uncoated ELISA KitRepresentative standard curve for bovine IL-4 ELISA

技术参数
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