| The ProQuantum Mouse IL-17A Immunoassay Kit is designed to provide quantitative measurements of mouse IL-17A in small sample volumes. Utilizing proximity-based amplification technology, the assay combines the analyte specificity of high-affinity antibody-antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a simple yet powerful next-generation protein quantitation platform. A user-friendly workflow combined with intuitive software for analytics enables sample-to-answer in just 2 hours.ProQuantum小鼠IL-17A免疫测定试剂盒旨在提供小样品量中小鼠IL-17A的定量测量。 该分析利用基于邻近的扩增技术，将高亲和力抗体-抗原结合的分析物特异性与实时PCR的信号检测和扩增功能结合在一起，从而实现了一个简单而强大的下一代蛋白质定量平台。 用户友好的工作流程与直观的分析软件相结合，可在2小时内完成从样品到答案的分析。ProQuantum immunoassays utilize a matched pair of target-specific antibodies, each conjugated to a DNA oligonucleotide. During antibody-analyte binding, the two DNA oligos are brought into close proximity, which allows for ligation of the two strands and subsequent creation of a template strand for amplification. This platform leverages the sensitivity and large dynamic range of Applied Biosystems TaqMan real-time PCR technology .ProQuantum免疫测定利用一对配对的靶标特异性抗体，每种抗体均与DNA寡核苷酸偶联。 在抗体-分析物结合过程中，两个DNA寡核苷酸紧密靠近，这允许两条链连接并随后产生模板链进行扩增。 该平台利用了Applied Biosystems TaqMan实时PCR技术的灵敏度和大动态范围。The assay workflow is fast and easy—2 steps in 2 hours. There are a total of 7 components in each kit (Figure 2). First, mix the antibody-conjugates, dilute the sample, and create the standard curve in a working plate. Then, using a multi-channel pipette, add the antibody-conjugates and sample (or standard) into the wells of a PCR plate and incubate for 1 hour. Combine the master mix and ligase and add to the wells of the PCR plate, then run the plate on any qPCR instrument. After the run is complete, import the results file into the ProQuantum software (cloud-based version at https://apps.thermofisher.com/apps/proquantum). Using this software, the data can be analyzed easily to obtain protein concentration values. The software allows you to set up standard curves, design plate layouts, apply a 5PL weighted algorithm, and obtain robust statistical group-wise comparisons.测定工作流程快速简便— 2小时内完成2个步骤。 每个套件中总共有7个组件（图2）。 首先，混合抗体-缀合物，稀释样品，并在工作板上创建标准曲线。 然后，使用多通道移液器，将抗体结合物和样品（或标准品）添加到PCR板的孔中，并孵育1小时。 合并预混液和连接酶，并添加到PCR板的孔中，然后在任何qPCR仪器上运行该板。 运行完成后，将结果文件导入ProQuantum软件（基于云的版本，网址为https://apps.thermofisher.com/apps/proquantum）。 使用此软件，可以轻松分析数据以获得蛋白质浓度值。 该软件可让您设置标准曲线，设计板布局，应用5PL加权算法并获得可靠的逐组统计比较。Target information 靶标信息Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).白介素-17A（IL-17A，CTLA-8）是CD4 + T细胞衍生的细胞因子，可促进细胞系中的炎症反应，并在类风湿性关节炎，哮喘，多发性硬化症，牛皮癣和移植排斥反应中升高。 IL-17A是一个32 kDa长的二硫键连接的同型二聚体，由136个氨基酸组成，是六种蛋白质家族（IL-17A-17F）的成员，并通过IL-17受体（IL-17R / CDw217）。活化的外周血CD4 + T细胞产生高水平的IL-17A同型二聚体，并且IL-17A还增强人成纤维细胞中细胞内粘附分子1（ICAM-1）的表达。特别地，人IL-17A还刺激上皮，内皮或成纤维细胞分泌IL-6，IL-8，G-CSF和PGE2。在人IL-17A存在的情况下，成纤维细胞可以维持CD34 +造血祖细胞的增殖并诱导成熟为嗜中性粒细胞。小鼠，大鼠和人IL-17A可以诱导小鼠基质细胞分泌IL-6，这表明所有同源物都可以识别小鼠受体。 IL-17A调节NF-κB和丝裂原激活的蛋白激酶的活性，刺激IL-6和环氧合酶2（PTGS2 / COX-2）的表达，并增强一氧化氮（NO）的产生。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。 |
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