FOXN3 Recombinant Polyclonal Antibody (1HCLC)/FOXN3重组多克隆抗体（1HCLC）

货号：711585

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Peptides corresponding to Human FOXN3 (aa 381-398,

物种：人

宿主：兔

抗体亚型：IgG

克隆号：1HCLC

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	2.5 µg x 10^6 cells(ChIP)；2 µg/mL(ICC)；2 µg/mL(IF)；1-2 µg/mL(WB)

产品详细信息This antibody is predicted to react with Monkey, Hors and CatRecombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.靶标信息FOXN3 is a member of the forkhead/winged helixtranscription factor family. Checkpoints are eukaryotic DNA damage-inducible cell cycle arrests at G1 and G2. Check point suppressor 1 suppresses multiple yeast checkpoint mutationsincluding mec1, rad9, rad53 and dun1 by activating aMEC1-independent checkpoint pathway. Alternative splicing isobserved at the locus, resulting in distinct isoforms.

数据：

FOXN3 Antibody (711585) in IFFor immunofluorescence analysis, Hep G2 cells were fixed and permeabilized for detection of endogenous FOXN3 using Anti- FOXN3 Recombinant Rabbit Polyclonal Antibody (Product # 711585, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of FOXN3 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of FOXN3. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. FOXN3 Antibody (711585) in WBWestern blot analysis was performed on Nuclear enriched extracts (30 µg lysate) of Jurkat (Lane 1), Hep G2 (Lane 2), KARPAS 299 (Lane 3), HT-29 (Lane 4), THP-1 (Lane 5), U-937 (Lane 6) and U-2 OS (Lane 7). The blots were probed with Anti-FOXN3 Recombinant Rabbit Polyclonal Antibody (Product # 711585, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 54 kDa band corresponding to FOXN3 protein was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106). FOXN3 Antibody (711585) in ChIPEnrichment of endogenous FOXN3 protein at specific gene loci using Anti-FOXN3 Recombinant Rabbit Polyclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-FOXN3 Recombinant Rabbit Polyclonal Antibody (Product # 711585, 5 µg) on sheared chromatin from 2 million HEPG2 cells using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG (1 µg) was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the different loci of the active MYC, PIM2 promoter region used as positive control target genes, and the region of the inactive SAT2 satellite repeat, used as negative control target gene. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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