IL-17A Monoclonal Antibody (eBio17B7), PE, eBioscience™/IL-17A 单克隆抗体 (eBio17B7), PE, eBioscience™

货号：12-7177-81

规格：50 µg

价格：1724

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

荧光染料：PE

类型:	一抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™
浓度：	0.2 mg/mL	用法：	0.125 µg/test(Flow)

产品详细信息

Description: The eBio17B7 antibody reacts with mouse and rat IL-17A with no recognition of IL-17F. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor.IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN gamma and IL-4, have each been found to negatively regulate the generation of these Th-17 cells.Applications Reported: The eBio17B7 antibody has been reported useful for intracellular staining for flow cytometric analysis.Applications Tested: This eBio17B7 antibody has been tested by intracellular staining and flow cytometric analysis of restimulated, Th17-polarized mouse splenocytes using the Intracellular Fixation and Permeabilization Buffer Set (cat. 88-8824) and protocol. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Staining has been successfully done using the Foxp3 buffer system (cat 00-5523).Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).

数据

IL-17A Antibody (12-7177-81) in FlowIntracellular staining of 10 day-Th17 polarized mouse splenocytes either unstimulated (left) or stimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) (Product # 00-4975-03) (right) with Anti-Mouse CD4 FITC (Product # 11-0042-82) and 0.125 µg of Anti-Mouse/Rat IL-17A PE using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Viable cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Frontiers in pharmacologyTraditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal Gamma-Delta T Cells.2.Evidence-based complementary and alternative medicine : eCAMBaicalin Downregulates RLRs Signaling Pathway to Control Influenza A Virus Infection and Improve the Prognosis.3.Frontiers in immunologyVibrio vulnificusRtxA Is a Major Factor Driving Inflammatory T Helper Type 17 Cell Responsesin vitroandin vivo.4.Proceedings of the National Academy of Sciences of the United States of AmericaIRF4 is essential for IL-21-mediated induction, amplification, and stabilization of the Th17 phenotype.5.Journal of immunology (Baltimore, Md. : 1950)Leishmania braziliensis infection induces dendritic cell activation, ISG15 transcription, and the generation of protective immune responses.

技术参数

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