GLI2 Recombinant Rabbit Monoclonal Antibody (9H25L4)/GLI2重组兔单克隆抗体（9H25L4）

货号：702013

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Protein corresponding to human GLI2 [aa1-aa332]

物种：人

宿主：兔

抗体亚型：IgG

克隆号：9H25L4

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	2.5 µg/1x10^6 cells(ChIP)；5 µg/mL(ICC)；5 µg/mL(IF)

产品详细信息This antibody is predicted to react with Monkey, Pig, Bovine, RabbitRecombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息Gli-2 belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.

数据：

GLI2 Antibody (702013) in IFFor immunofluorescence analysis, NTERA-2 cells were fixed and permeabilized for detection of endogenous GLI-2 using Anti- GLI-2 Recombinant Rabbit Monoclonal Antibody (Product # 702013, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of GLI-2 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating Nuclear localization of GLI-2. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. GLI2 Antibody (702013) in ChIPEnrichment of endogenous GLI-2 protein at specific gene loci using Anti-GLI-2 Recombinant Rabbit Monoclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-GLI-2 Recombinant Rabbit Monoclonal Antibody (Product # 702013, 5 µg) on sheared chromatin from 2 million NTERA-2 cells using the MAGnify ChIP system kit (Product # 49-2024). Normal Rabbit IgG (1 µg) was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the promoter of the GLI-1 used as positive control target genes, and the promoter region of the E2F3, SAT2 satellite repeat used as negative control target gene. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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