Glucocorticoid Receptor Polyclonal Antibody/糖皮质激素受体多克隆抗体

货号：PA1-512

规格：50 µg

价格：5468

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Synthetic peptide corresponding to residues C(245)

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	Assay dependent(ChIP)；Assay dependent(GS)；Assay dependent(ICC)；1:20(IF)；1 µg/mL(IHC (F))；5 µg/mL(IP)；5 µg/mL(WB)

产品详细信息PA1-512 detects glucocorticoid receptor (GR) from human, mouse and rat tissues.PA1-512 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunofluorescence, ChIP and gel shift procedures. By Western blot, this antibody detects an ~97 kDa protein representing GR as well as two other unidentified proteins at ~135 to ~145 kDa from rat liver extract. These cross reacting proteins are not believed to be GR or GR precursors as they donot bind [3H]dexamethasone mesylate. Immunohistochemical staining of GR in rat hippocampus using PA1-512 results in staining primarily in the nucleus. PA1-512 does not disrupt receptor-DNA interactions.The PA1-512 immunogen is a synthetic peptide corresponding to residues C(245) K P L I L P D T K P K I K D(259) of human GR. The PA1-512 immunizing peptide (Cat. # PEP-002) is available for use in for neutralization and control experiments.Reconstitute with PBS.靶标信息Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance.

数据：

Glucocorticoid Receptor Antibody (PA1-512) in IFImmunofluorescent analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Polyclonal Antibody (59) (Product # PA1-512) shows staining in Hela Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor (Product # PA1-512) at a dilution of 1:100 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552for GAR,Product # 35503for GAM). Images were taken at 60X magnification. Glucocorticoid Receptor Antibody (PA1-512) in IFImmunofluorescent analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Polyclonal Antibody (59) (Product # PA1-512) shows staining in A2058 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor (Product # PA1-512) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552for GAR,Product # 35503for GAM). Images were taken at 60X magnification. Glucocorticoid Receptor Antibody (PA1-512)Genes and development 2003 -Published figure using Glucocorticoid Receptor polyclonal antibody (Product # PA1-512)

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参考文献：

1. Proceedings of the National Academy of Sciences of the United States of AmericaTumor suppressor protein (p)53, is a regulator of NF-kappaB repression by the glucocorticoid receptor.2. The Journal of biological chemistryFunctional modulation of the glucocorticoid receptor and suppression of NF-kappaB-dependent transcription by ursodeoxycholic acid.3. EndocrinologyImmunocytochemical localization of the glucocorticoid receptor in rat brain, pituitary, liver, and thymus with two new polyclonal antipeptide antibodies.4. Molecular endocrinology (Baltimore, Md.)Progesterone receptor directly inhibits β-casein gene transcription in mammary epithelial cells through promoting promoter and enhancer repressive chromatin modifications.5. Journal of immunology (Baltimore, Md. : 1950)Cross talk between glucocorticoid and estrogen receptors occurs at a subset of proinflammatory genes.

技术参数

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