p53 Monoclonal Antibody (PAb 1801)/p53单克隆抗体（PAb 1801）

货号：MA5-11296

规格：500 µL

价格：4880

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Recombinant Human p53 protein

物种：人

宿主：小鼠

抗体亚型：其它

克隆号：PAb 1801

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.2 mg/mL	用法：	5 µg(ChIP)；2 µg/mL(IP)；0.5-2 µg/mL(WB)

产品详细信息MA5-11296 targets p53 in FACS, IP, and WB applications and shows reactivity with Human samples.The MA5-11296 immunogen is recombinant Human p53 protein.靶标信息The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.

数据：

p53 Antibody (MA5-11296) in WBWestern blot analysis was performed using nuclear enriched extracts (30 µg lysate) of MDA-MB-231 (Lane 1) and T-47D (Lane 2). The blots were probed with Anti-p53 Mouse Monoclonal Antibody (Product # MA5-11296, 0.5-2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A 53 kDa band corresponding to p53 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106). p53 Antibody (MA5-11296) in ChIPEnrichment of endogenous p53 Protein at specific gene loci using Anti-p53 Mouse Monoclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-p53 Mouse Monoclonal Antibody (Product # MA5-11296, 5 µg) on sheared chromatin from 2 million MCF-7 cells, UV treated for 30 minutes using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Mouse IgG was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the promoter of active CDKN1A, CMBL gene, used as positive control targets, and the SAT2, used as negative control target. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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参考文献：

1. Cellular and molecular life sciences : CMLSMitochondrial ferritin, a new target for inhibiting neuronal tumor cell proliferation.2. PloS oneChimeras of p14ARF and p16: functional hybrids with the ability to arrest growth.3. International journal of oncologyIncrease in clusterin forms part of the stress response in Hodgkin's lymphoma.4. International journal of cancerResveratrol-mediated apoptosis of hodgkin lymphoma cells involves SIRT1 inhibition and FOXO3a hyperacetylation.5. Journal of clinical pathologyImmunohistochemical analysis of candidate gene product expression in the duodenal epithelium of children with coeliac sprue.

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