STAT2 Polyclonal Antibody/STAT2多克隆抗体

货号：44-362G

规格：100 µL

价格：4692

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：The antiserum was produced against a chemically sy

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：		用法：	10 µL(ChIP)；1:250(ICC)；1:250(IF)；1:10-1:100(IHC (P))；1:200-1:2000(IP)；1:1000(WB)

靶标信息STAT2 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus.

数据：

STAT2 Antibody (44-362G) in IFImmunofluorescent analysis of STAT2 was done on 70% confluent log phase HeLa cells treated with IFN-gamma (100 ng/mL for 30 min). The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with STAT2 Rabbit polyclonal Antibody (Product # 44-362G) at 1:250 dilution in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (Product # A12381). Panel d is a merged image showing nuclear and cytoplasmic localization upon treatment. Panel e showing cytoplasmic localization in untreated HeLa cells. Panel f is a no primary antibody control. The images were captured at 20X magnification. STAT2 Antibody (44-362G) in WBWestern blot analysis of STAT2 was performed by loading 20 µg of Caco-2 (lane1), PC-3 (lane2), MDA-MB-231 (lane3), Raji (lane4) and A431 (lane5) cell lysate using Novex®NuPAGE® 12 % Bis-Tris gel (Product # NP0341BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk at 4°C overnight. STAT2 was detected at ~113 kDa using STAT2 Rabbit Polyclonal Antibody (Product # 44-362G) at 1:1000 dilution in 5% skim milk for 3 hour at room temperature on a rocking platform. Goat Anti-Rabbit IgG - HRP Secondary Antibody (G21234) at 1:5000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

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