IFN gamma Bovine ELISA Kit/ 牛IFNγ ELISA试剂盒
货号：KBC1231
规格：96 tests
价格：7030
产品类型：Platinum ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 半定量 |
| 检测范围 | 半定量 |
样本类型/体积 | 上清液 100 μL 血浆 100μL |
操作时间 | 1 hr 20 min |
测试时间 | 2 hr 15 min |
Homogenous (no wash) | No |
| 批间变异系数（CV） | 2.6% |
| 批内变异系数（CV） | 3% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 标准稀释液 - 孵育缓冲液 - HRP-共轭检测抗体 - 洗涤液 - 显色剂 - 终止液 - 对照 |
储存温度 | 2-8℃ |
| Protein name | IFN gamma |
| Protein aliases | BoIFNG, IFN-gamma, Interferon gamma, interferon gamma type 2, interferon-gamma |
物种（Tested） | 牛 |
试剂盒类型 | Sandwich ELISA Kit |
标记（染料） | HRP |
| 检测抗体偶联物 | HRP |
| Gene aliases | BOS_5228, IFNG |
| Gene ID | (Bovine) 281237 |
| Gene symbol | IFNG |
| UniProt ID | (Bovine) P07353 |
The Bovine Interferon gamma (Bov IFN-gamma) ELISA semi-quantitates Bov IFN-gamma in plasma or cell culture medium. The assay will exclusively recognize natural Bov IFNγ.牛干扰素γ（牛IFN-γ）ELISA可半定量血浆或细胞培养基中的牛IFN-γ。 该检测试剂盒将专门识别天然牛IFNγ。Principle of the method 方法原理The Bov IFNγ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.牛IFNγ固相夹心ELISA（酶联免疫吸附测定）用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品或对照添加到这些孔中，并与固定的（捕获）抗体结合。 通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.IFNγ（干扰素γ，II型干扰素）是一种巨噬细胞活化因子，是一种免疫干扰素，主要由T淋巴细胞和自然杀伤细胞响应抗原，促分裂原，葡萄球菌肠毒素B，植物血凝素和其他细胞因子而产生。 IFNγ是由两个146个氨基酸亚基组成的二聚体蛋白。 IFNγ是一种糖蛋白，功能上以约45 kDa的同型二聚体形式存在。在SDS-PAGE上，由于差异糖基化，IFNγ出现了25、20和15.5 kDa较小的条带。 IFNγ同二聚体的生物活性是高度物种特异性的。人IFNγ与小鼠无交叉反应。 IFNγ功能包括以下各项：抗病毒活性，肿瘤抗增殖活性，I类和II类MHC的诱导，巨噬细胞活化以及B淋巴细胞增强的免疫球蛋白分泌。 IFNγ参与细胞因子的调节，并与其他细胞因子协同作用。 IFNγ的激活是通过IFNγ受体I和II的结合并激活JAK-STAT途径进行的。 IFNγ与IFNα或IFNβ没有任何同源性，但是人IFNγ与小鼠IFNγ具有约40％的序列同源性。 IFNγ被IL2，FGF碱性蛋白，EGF上调，而维生素D3或DMN被下调。 IFNγ基因突变与再生障碍性贫血有关。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。
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