STAT6 Recombinant Rabbit Monoclonal Antibody (21HCLC)/STAT6重组兔单克隆抗体(21HCLC)
货号:701110
规格:100 µg
价格:4809
产品类型:抗体和染料
品牌:Thermo Fisher
抗原:Peptide corresponding to amino acids 630–638 of human singal transducer and activator of transcription 6
物种:人/小鼠
宿主:兔
抗体亚型:IgG
克隆号:21HCLC
荧光染料:其它
类型: | 单抗 | 同型对照: | |
浓度: | 0.5 mg/mL | 用法: | 3 µg(ChIP);1-3 µg/mL(ICC);1-3 µg/mL(IF);1:50-1:200(IHC (P));1-3 µg/mL(WB) |
产品详细信息Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息STAT6 protein is a transcription factor activated by cytokines, particularly interleukin-4 and IL13. (Stat6-/-) were found to be deficient in IL-4-mediated functions including Th2 helper T-cell differentiation, expression of cell surface markers, T-cell proliferation, immunoglobulin class switching to IgE, and partial loss of IL-4 mediated proliferation. STAT6 mRNA has been detected in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung and placenta. STAT6 is critically involved in Th2 immune response. STAT6 is crucial for IL-4-mediated biological responses, and naturally in two isoforms: STAT6a and STAT6c.
数据: |
STAT6 Antibody (701110) in IFImmunofluorescent analysis of STAT6 in HeLa cells using a STAT6 recombinant rabbit monoclonal antibody (Product # 701110) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing cytoplasmic localization of STAT6. STAT6 Antibody (701110) in IHC (P)Immunohistochemistry analysis of STAT6 showing staining in the cytoplasm and nucleus of paraffin-embedded human thymus tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a STAT6 monoclonal antibody (Product # 701110) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. |
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