STAT5 beta Monoclonal Antibody (ST5b-10G1)/STAT5 beta单克隆抗体（ST5b-10G1）

货号：13-5300

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：A peptide corresponding to the unique carboxy-term

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：其它

克隆号：ST5b-10G1

荧光染料：其它

类型:	单抗	同型对照：
浓度：		用法：	5 µg/mL(ChIP)；Assay Dependent(ELISA)；Assay Dependent(GS)；1:250(ICC)；1:250(IF)；1:20-1:200(IHC (P))；Assay Dependent(IP)；1:250(WB)

靶标信息The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL.

数据：

STAT5 beta Antibody (13-5300) in IFImmunofluorescent analysis of STAT5 beta was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with STAT5 beta Mouse Monoclonal Antibody (Product # 13-5300) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (Product # A12381). Panel d is a merged image showing cytoplasmic localization. Panel e shows no primary antibody control. The images were captured at 20X magnification. STAT5 beta Antibody (13-5300)PloS one 2015 -Figure 6 Binding activity of two potential STAT5 binding sites located within the CSN1S1 distal upstream region. (A) Representative EMSA. Mammary nuclear extract (22ug) from rabbits in early lactation were incubated with the indicated labelled 32 P probes in the presence or absence of STAT5a or STAT5b antibodies (1 or 0.5 ug, respectively). Complexes were analysed on non denaturating 5% acrylamide gels in 0.25xTBE. The lower panel is an overexposed image of the upper one to show the weak interaction between D1 and nuclear extracts and the supershifts with STAT5 antibodies. (B) Competition assays with increasing amounts of unlabelled C, D2, D1 and D1m using 32 P-C or 32 P-D2, are indicated in each graph. Results are expressed as a percentage of the radioactivity bound in the absence of unlabelled oligonucleotides and plotted as a function of the log (ng oligonucleotide) in the reaction.

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参考文献：

1. PloS oneInhibition of the JAK/STAT Signaling Pathway in Regulatory T Cells Reveals a Very Dynamic Regulation of Foxp3 Expression.2. The Journal of biological chemistryIdentification of human STAT5-dependent gene regulatory elements based on interspecies homology.3. PloS oneDNA methylation and transcription in a distal region upstream from the bovine AlphaS1 casein gene after once or twice daily milking.4. The Biochemical journalHormone-induced modifications of the chromatin structure surrounding upstream regulatory regions conserved between the mouse and rabbit whey acidic protein genes.5. PloS oneIdentification of STAT5A and STAT5B target genes in human T cells.

技术参数

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