Histone H2A.Z Recombinant Polyclonal Antibody/组蛋白H2A.Z重组多克隆抗体

货号：711485

规格：100 µg

价格：4553

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Peptides corresponding to human H2A.Z [1) aa109 -a

物种：人/小鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	2.5 µg/1x10^6 cells(ChIP)；1 µg/mL(WB)

产品详细信息This antibody is predicted to react with Monkey, Rat, Pig, SheepRecombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.靶标信息Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

数据：

Histone H2A.Z Antibody (711485) in WBWestern blot analysis was performed on nuclear extract (20 µg lysate) of U-2 OS (Lane 1), HeLa (Lane 2), COLO-205 (Lane 3), K-562 (Lane 4), HCT 116 (Lane 5) Jurkat (Lane 6), HT-29 (Lane 7) and NIH/3T3 (Lane 8). The blots were probed with Anti-H2A.Z Recombinant Rabbit Polyclonal Antibody (Product # 711485, 1 µg/mL). A monomeric form of 17 kDa and tetramaric form of 68 kDa band corresponding to H2A.Z was observed across the cell lines tested. The blots were detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:5000 dilution). Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106). Histone H2A.Z Antibody (711485) in ChIPEnrichment of endogenous H2A.Z protein at specific gene loci using Anti-H2A.Z Recombinant Rabbit Polyclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-H2A.Z Recombinant Rabbit Polyclonal Antibody (Product # 711485, 5 µg) on sheared chromatin from 2 million HeLa cells using the MAGnify ChIP system kit (Product # 49-2024). Normal Rabbit IgG (1 µg) was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the promoters of the active C-FOS, PABPC1, GAPDH promoter region used as positive control target genes, and the region of the inactive GAPDH Intron, SAT2 satellite repeat, used as negative control target gene. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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