Histone H2B Monoclonal Antibody (C.362.2)/组蛋白H2B单克隆抗体（C.362.2）

货号：MA5-14835

规格：100 µL

价格：4880

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Synthetic peptide corresponding to the carboxy-ter

物种：其它

宿主：小鼠

抗体亚型：其它

克隆号：C.362.2

荧光染料：其它

类型:	单抗	同型对照：
浓度：		用法：	1.5 µg/1x10^6 cells(ChIP)；1:1,000(WB)

产品详细信息It is not recommended to aliquot this antibody.This antibody is not cross-reactive with other histones.靶标信息Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The nucleosomes wrap further and compact DNA into chromotin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones play a central role in transciption regulation, DNA repair, DNA replication, and chromosomal stability. H2B has a broad antibacterial activity.

数据：

Histone H2B Antibody (MA5-14835) in WBWestern blot analysis of Histone H2B was performed by loading 15 µg of the indicated whole cell or tissue lysates per well onto an SDS-PAGE gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% milk for one hour at room temperature. The membrane was probed with a Histone H2B monoclonal antibody (Product # MA5-14835) at a dilution of 1:2000 overnight at 4C, washed in TBST, and probed with an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:2000 for one hour at room temperature. Detection was performed using ECL substrate (Product # 32209). Data courtesy of the Innovators Program. Histone H2B Antibody (MA5-14835) in ChIPEnrichment of endogenous Histone H2B protein at specific gene loci using Anti-Histone H2B Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-Histone H2B Monoclonal Antibody (Product # MA5-14835, 3 µg) on sheared chromatin from 2 million HeLa treated cells using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over the PABPC1 gene (inactive) and MYOD, SATA (active). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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